The TruSight Oncology 500 High-Throughput assay is built on the foundation of the TruSight Oncology 500 assay, with flexibility for higher sample throughput and batching options.
Consolidate Multiple Biomarker Assays into One
Streamlined Sample-to-Results Workflow
High Sample Throughput
Achieve Highly Confident Results
Enable In-House Comprehensive Genomic Profiling
*Automation available on the Beckman Coulter i-Series or the Hamilton Microlab STAR. Contact Illumina Sales for more information.
All three TruSight Oncology 500 assays are designed to identify key biomarkers in guidelines and clinical trials.
Assess key cancer biomarkers from FFPE tumor tissue with the TruSight Oncology 500 Assay.
Batch up to 192 samples at a time while using the same biomarkers and tissue type as the TruSight Oncology 500 assay.
Analyze circulating tumor DNA from plasma with the TruSight Oncology 500 ctDNA assay.
TruSight Oncology 500 High-Throughput | TruSight Oncology 500 | TruSight Oncology 500 ctDNA | |
---|---|---|---|
Cancer Type | Pan-Cancer | Pan-Cancer | Pan-Cancer |
Content Specifications | Targeted selection of DNA from 523 genes of interest, and RNA from 55 genes, for a total of 1.94Mb panel size. • Guideline Coverage: Broad coverage of key guidelines for multiple solid tumor types • Clinical Trials Coverage: Over 1,000 clinical trials • Immuno-oncology Biomarker Coverage: Biomarkers TMB and MSI included; also inclusive of HLA regions, POLE1 and POLD1* |
Targeted selection of DNA from 523 genes of interest, and RNA from 55 genes, for a total of 1.94Mb panel size. • Guideline Coverage: Broad coverage of key guidelines for multiple solid tumor types • Clinical Trials Coverage: Over 1,000 clinical trials • Immuno-oncology Biomarker Coverage: Biomarkers TMB and MSI included; also inclusive of HLA regions, POLE1 and POLD1* • TruSight Oncology 500 HRD** kit content includes coverage of ~25,000 SNP's to assess homologous recombination deficiency through a comprehensive genomic instability score (LOH+TAI+LST), powered by Myriad Genetics • TruSight Oncology 500 HRD** is an optional add-on kit to TruSight Oncology 500 **Not available in Japan |
Targeted selection of 523 genes (full coding sequence) for a total of 1.94Mb panel size. • Guideline Coverage: Broad coverage of key guidelines for multiple solid tumor types • Immuno-oncology Biomarker Coverage: TMB and MSI* |
Hands-On Time | Manual: ~10.5 hrs Automated: ~2.5 hrs |
Manual: ~10.5 hrs Automated: ~2.5 hrs (TruSight Oncology 500 only) |
Manual: ~10.5 hrs Automated: N/A |
Input Quantity | 40 ng DNA, 40-80 ng RNA (need at least 2 mm3 FFPE tissue) | 40 ng DNA, 40 ng RNA | |
Method | Target Enrichment, Target Enrichment, Targeted DNA Sequencing, Targeted RNA Sequencing | Target Enrichment, Target Enrichment, Targeted DNA Sequencing, Targeted RNA Sequencing | Target Enrichment, Target Enrichment, Targeted DNA Sequencing |
Nucleic Acid Type | RNA, DNA | RNA, DNA | DNA |
Sample Throughput | 16–192 samples per flow cell | ||
Specialized Sample Types | FFPE Tissue | FFPE Tissue | Blood, Circulating Tumor DNA |
Species Category | Human | Human | Human |
System Compatibility | NovaSeq 6000 | NextSeq 500, NextSeq 550, NextSeq 550Dx in Research Mode | NovaSeq 6000 |
Technology | Sequencing | Sequencing | Sequencing |
Variant Class | Copy Number Variants (CNVs), Gene Fusions, Insertions-Deletions (indels), Single Nucleotide Variants (SNVs), Transcript Variants | Copy Number Variants (CNVs), Gene Fusions, Insertions-Deletions (indels), Single Nucleotide Variants (SNVs), Transcript Variants | Copy Number Variants (CNVs), Gene Fusions, Insertions-Deletions (indels), Single Nucleotide Variants (SNVs), Somatic Variants |
*Based on Pierian Clinical knowledgebase, as of March 2020
Simplifying the process to identify applicable variants in oncology samples
Brochure | PDF 2 MB
Optimizing RNA input to detect gene fusions with TruSight Oncology 500 High-Throughput
Technical Note | PDF < 1 MB
Data analysis and reporting for the TruSight Oncology 500 portfolio
Product Information Sheet | PDF 1 MB
Data Sheet | PDF | 7 versions
DRAGEN TruSight Oncology 500 Analysis Software v1.1 Documentation
TruSight Oncology 500 v1.0 Local App Documentation
TruSight Oncology 500 v2.0 Local App Documentation
TruSight Oncology 500 v2.1 Local App Documentation
DRAGEN TruSight Oncology 500 Analysis Software v2.1 Documentation
TruSight Oncology 500 High Throughput Reference Guide Documentation
DRAGEN TruSight Oncology 500 Analysis Software v1.1 on ICA Documentation
DRAGEN TruSight Oncology 500 Analysis Software v2.1.1 on ICA Documentation
TruSight Oncology 500 High Throughput Consumables & Equipment Documentation
TruSight Oncology 500 High Throughput Checklist Documentation
TruSight Oncology 500 v2.2 Local App Documentation
Custom Protocol Selector
Generates customized, end-to-end instructions