We recommend ordering the new Illumina DNA/RNA UD Indexes Sets A-D (20091654, 20091656, 20091658, 20091660). The IDT for Illumina DNA/RNA UD Indexes Sets A-D (20027213, 20027214,20042666, 20042667) are obsolete and the last order date is January 30, 2025, as announced in PON2023-1440.
The TruSight Hereditary Cancer Panel content was selected based on feedback from key opinion leaders on genetic risk assessment research.
TruSight Hereditary Cancer Panel was created as a modular panel that uses Illumina DNA Prep with Enrichment for library preparation.
Indexes are available for up to 384 samples to allow for flexible batch sizes and scalability across all Illumina benchtop sequencers (iSeq 100, MiSeq, MiniSeq, and NextSeq).
The Illumina DNA Prep with Enrichment library prep workflow uses enzyme-based DNA fragmentation, removing the instrument cost and need for mechanical fragmentation. Illumina DNA Prep with Enrichment has been qualified with multiple automation systems to allow for a user-friendly workflow.
TruSight Hereditary Cancer Panel | TruSight Cancer | AmpliSeq for Illumina BRCA Panel | |
---|---|---|---|
Assay Time | ~6.5 hours | 1.5 days | 5 hours (library prep only; doesn't include library quantification, normalization, or pooling time) |
Content Specifications | Targets 113 cancer risk-associated genes | Fixed set of probes that enrich for 94 genes and 284 SNPs associated with common and rare cancers. | Exonic regions and the flanking intronic sequences of the BRCA1 and BRCA2 |
Hands-On Time | 2 hours | 5 hours | < 1.5 hours |
Input Quantity | 50 ng | 50 ng DNA | 1–100 ng (10 ng recommended per pool) |
Mechanism of Action | Bead-bound transposomes and hybrid-capture chemistry | Multiplex PCR | |
Method | Target Enrichment, Target Enrichment, Targeted DNA Sequencing | Target Enrichment, Target Enrichment, Targeted DNA Sequencing | Amplicon Sequencing, Targeted DNA Sequencing |
Nucleic Acid Type | DNA | DNA | DNA |
Technology | Sequencing | Sequencing | Sequencing |
Understanding the Polygenicity of Cancer.
icommunity_articles | PDF 4 MB
Data Sheet | HTML | 7 versions
Data Sheet | HTML | 5 versions
Analyze germline CNVs with TruSight Hereditary Cancer Panel
Technical Note | PDF 1 MB
Data Sheet | PDF | 5 versions
Data Sheet | PDF | 5 versions
Custom Protocol Selector
Generates customized, end-to-end instructions
All Illumina DNA RNA UD Indexes Support All Illumina DNA Prep with Enrichment Support