Instruments

iSeq 100 Sequencing System applications and methods

Multi-application flexibility

Flexible read lengths allow an easy transition from small-scale DNA and RNA sequencing projects

iSeq 100 sequencing reagents

Key applications and methods

Targeted DNA sequencing allows researchers to focus on a select set of genes or relevant gene regions using fixed panels of expert-selected content or custom, user-defined gene panels. By focusing the power of next-generation sequencing (NGS), targeted sequencing enables cost-effective and systematic variant detection with high accuracy and reproducibility.

Read application note: amplicon cancer panels

1
Prep

AmpliSeq for Illumina Cancer HotSpot Panel v2

Targeted panel investigating hotspot regions of 50 genes with known associations to cancer.

AmpliSeq for Illumina Focus Panel

Targeted DNA and RNA panel investigating 52 genes with known relevance to solid tumors.

AmpliSeq for Illumina BRCA Panel

Targeted panel investigating somatic and germline variants in BRCA1 and BRCA2.

AmpliSeq for Illumina Immune Response Panel

Targeted RNA expression panel investigating 395 genes involved in tumor-immune system interactions.

AmpliSeq for Illumina Custom DNA Panel

Targeted custom panel optimized for specific targets or genomic content of interest.

2
Sequence

iSeq 100 Reagents

Reagents include a flow cell and prefilled, ready-to-use cartridges containing clustering and sequencing reagents to support cycling runs on the iSeq 100 System.

3
Analyze

Local Run Manager (DNA Amplicon Analysis Module)

An on-premises software solution for creating sequencing runs, monitoring run status, and analyzing data.

BaseSpace Sequence Hub

The Illumina genomics cloud-computing environment for NGS data analysis and management.

Find targeted DNA sequencing education

Bacterial sequencing enables comprehensive characterization of bacterial genomes, including antibiotic resistance, molecular epidemiology, and virulence. Using NGS, sequencing of bacterial genomes can be done quickly while producing highly accurate data. 

Read surveillance application note

1
Library prep

Illumina DNA Prep

Fast, integrated workflow for a wide range of applications, including sequencing whole human genomes, amplicons, plasmids, and microbial genomes.

2
Sequence

iSeq 100 Reagents

Reagents include a flow cell and prefilled, ready-to-use cartridges containing clustering and sequencing reagents to support cycling runs on the iSeq 100 System.

3
Analyze

Local Run Manager (Resequencing Analysis Module)

An on-premises software solution for creating sequencing runs, monitoring run status, and analyzing data.

BaseSpace Sequence Hub

The Illumina genomics cloud-computing environment for NGS data analysis and management.

Find bacterial sequencing education

Viral sequencing is a fast and effective tool for characterizing viral agents, studying viral variability, and identifying genetic markers associated with virulence. 

Read application note: pan-viral sequencing

Read customer story: Ebola virus sequencing

1
Library prep

Illumina DNA Prep

Fast, integrated workflow for a wide range of applications, including sequencing whole human genomes, amplicons, plasmids, and microbial genomes.

Viral Surveillance Panel

Targeted RNA panel investigating 66 viruses that are of high risk to public health.

2
Sequence

iSeq 100 Reagents

Reagents include a flow cell and prefilled, ready-to-use cartridges containing clustering and sequencing reagents to support cycling runs on the iSeq 100 System.

3
Analyze

Local Run Manager (Resequencing Analysis Module)

An on-premises software solution for creating sequencing runs, monitoring run status, and analyzing data.

BaseSpace Sequence Hub

The Illumina genomics cloud-computing environment for NGS data analysis and management.

Find viral sequencing education

Resource spotlight

Methods guide for microbial whole-genome sequencing

Sequencing entire bacterial, viral, and other microbial genomes is important for generating accurate reference genomes, microbial identification, and comparative genomic studies. Learn more about microbial whole-genome sequencing and the Illumina products that can make it happen.

Access guide

More applications and methods

16S metagenomic sequencing

Sequence the 16S ribosomal RNA (rRNA) gene with a culture-free method to identify and compare bacteria from complex, difficult-to-study microbiomes or environments.

Learn more about 16S metagenomics
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Quality control

Perform quality control (QC) applications for bioproduction studies or assess the quality of a sequencing library before committing it to a full-scale run.

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Read customer interview

QC and rebalancing of single-cell libraries

Evaluate the quality and enable accurate rebalancing of multiplexed single-cell gene expression libraries before committing to a full-scale run.

Read application note
Small whole-genome sequencing

Sequence the entire genome of a bacterium, virus, or other microbe for applications in public health, infectious disease surveillance, molecular epidemiology studies, and environmental metagenomics.

Learn more about small WGS
Targeted gene sequencing

Select and sequence specific genes or genomic regions of interest using predesigned or custom designed panels for gene profiling studies.

Learn more about targeted gene sequencing
Read application note
Targeted RNA sequencing

Select and sequence specific transcripts of interest for gene expression profiling studies.

Learn more about targeted RNA-Seq

How others use this instrument

See what’s possible with the iSeq 100 System

The time is now for microbiome studies

Whole-genome shotgun sequencing and transcriptomics provide researchers and pharmaceutical companies with data to refine drug discovery and development.