Key Applications and Methods
Targeted DNA sequencing allows researchers to focus on a select set of genes or relevant gene regions, enabling cost-effective and systematic variant detection. Targeted resequencing on the iSeq 100 System provides highly accurate and reproducible data with a streamlined workflow. The iSeq 100 System supports various NGS study designs, including fixed panels of expert-selected content and custom, user-defined gene panels.
Application note:
Prep
1.5 hours total / 15 minutes hands-on
AmpliSeq for Illumina Cancer HotSpot Panel v2
Targeted panel investigating hotspot regions of 50 genes with known associations to cancer.
AmpliSeq for Illumina Focus Panel
Targeted DNA and RNA panel investigating 52 genes with known relevance to solid tumors.
AmpliSeq for Illumina BRCA Panel
Targeted panel investigating somatic and germline variants in BRCA1 and BRCA2.
AmpliSeq for Illumina Immune Response Panel
Targeted RNA expression panel investigating 395 genes involved in tumor-immune system interactions.
AmpliSeq for Illumina Custom DNA Panel
Targeted custom panel optimized for specific targets or genomic content of interest.
Sequence
19 hours total / 5 minutes hands-on
iSeq 100 Reagents
iSeq 100 Reagents bring the power of Illumina NGS to labs of all sizes.
Data Analysis
1–2 hours
Local Run Manager (DNA Amplicon Analysis Module)
Automatically performs on-instrument data analysis.
BaseSpace Sequence Hub
For downstream data analysis and storage.
Microbial whole-genome sequencing is an important tool for mapping genomes of novel organisms, finishing genomes of known organisms, or comparing genomes across multiple samples. Sequencing the entire microbial genome is important for generating accurate reference genomes, microbial identification, and other comparative genomic studies.
Application note:
Prep
1.5 hours total / 15 minutes hands-on
Illumina DNA Prep
A fast, integrated workflow for a wide range of applications, from human whole-genome sequencing to amplicons, plasmids, and microbial species.
Sequence
19 hours total / 5 minutes hands-on
iSeq 100 Reagents
iSeq 100 Reagents bring the power of Illumina NGS to labs of all sizes.
Data Analysis
1–2 hours
Local Run Manager (Resequencing Analysis Module)
Automatically performs on-instrument data analysis. Aligns reads and performs variant analysis for whole-genome sequencing of small genomes.
BaseSpace Sequence Hub
For downstream data analysis and storage.
Viral whole-genome sequencing is an important tool for mapping genomes of novel organisms, finishing genomes of known organisms, or comparing genomes across multiple samples. Viral and other microbial RNA-Seq experiments enable annotation and quantification of comprehensive microbial transcripts.
Prep
1.5 hours total /15 minutes hands-on
Illumina DNA Prep
A fast, integrated workflow for a wide range of applications, from human whole-genome sequencing to amplicons, plasmids, and microbial species.
Sequence
19 hours total / 5 minutes hands-on
iSeq 100 Reagents
iSeq 100 Reagents bring the power of Illumina NGS to labs of all sizes.
Data Analysis
1–2 hours
Local Run Manager (Resequencing Analysis Module)
Automatically performs on-instrument data analysis. Aligns reads and performs variant analysis for whole-genome sequencing of small genomes.
BaseSpace Sequence Hub
For downstream data analysis and storage.
The Time is Now for Microbiome Studies
Dissecting host—microbial relationships gives researchers and pharmaceutical companies data to refine drug discovery.
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More Applications and Methods
Library QC
Assess the quality and composition of your pooled NGS library on the iSeq 100 before committing to a full scale run on higher throughput sequencing systems.
QC and Rebalancing of Single-Cell Libraries
Evaluate quality and enable accurate rebalancing before high-depth runs on the NextSeq 550 or NovaSeq 6000 Systems.
Gene Expression Analysis with Targeted RNA-Seq
Targeted RNA sequencing focuses on specific transcripts of interest. It is used to analyze gene expression and identify fusion genes.
16S rRNA Sequencing
A culture-free method to identify and compare bacteria from complex microbiomes or environments that are difficult to study.
Targeted Gene Panels
Targeted gene sequencing panels contain defined probe sets focused on specific genes of interest. Both predesigned and custom panels are available.
Small Whole-Genome Sequencing
Small genome sequencing provides comprehensive analysis of bacterial, viral, and other microbial genomes for epidemiology and disease studies.