Instruments

Ordering the iSeq 100 Sequencing System

Order an iSeq 100 System today. Speak to a representative for information on pricing, availability, and more.

The iSeq 100 System makes next-generation sequencing (NGS) easier and more affordable than ever. With high-accuracy data and fast turnaround times, flexibility for a range of applications, and a simple, streamlined workflow, the iSeq 100 System is a powerful and versatile sequencing system positioned to help labs of all sizes advance their research. 

We are making the iSeq 100 Sequencing System obsolete. It will be available for order until September 30, 2025. We will continue to provide full system support through December 31, 2029. For more information, refer to PON2025-1657. The MiSeq i100 Series is the recommended alternative.

Documentation

Product literature
Support documentation

Resources

iSeq 100 Sequencing System: Introduction

This support webinar discusses key features of the iSeq 100 System, Illumina technologies that power the iSeq 100 System, the streamlined DNA-to-data workflow, and the troubleshooting path when working with Technical Support.

iSeq 100 Sequencing System—a solution for fast and efficient low-throughput sequencing for virtually any lab

Explore our smallest and most cost-effective sequencing system ever. Join Bellal Moghis and Gary Schroth as they introduce the iSeq 100 System. They’ll walk through installation and review applications and methods. Find out how you can make the most of your new lab partner.

How to start a sequencing run on the iSeq 100 System

See how easy it is to use the iSeq 100 System with this brief demonstration.

Find the right library prep kit

Library preparation is a required sequencing step. Use our selection tool to determine the best library prep kit for your needs based on your application, method, species and more.

Illumina resources and tools

Identify the best approach, manage your workflow, purchase what you need, and get support.

How do I prepare for my Illumina instrument delivery?

In this video, an Illumina Field Applications Scientist discusses what to expect during the preinstallation, shipping, and delivery stages. We’ll highlight important steps in the process and introduce you to available resources.

Instrument (1)

iSeq 100 System

20021532

Includes one iSeq 100 System, with mounted 10-inch touch screen monitor, one system test kit, on-instrument cluster generation, onboard bioinformatics capabilities, and 12 months warranty (including parts). The system is self-installable by the customer and does not include installation services. Qualification services, instrument service plans, and reagent kits sold separately.

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Reagents (3)

iSeq 100 i1 Reagent v2 (300-cycle)

20031371

Includes one i1 flow cell and one pre-filled cartridge containing clustering and sequencing reagents to support a 300-cycle run on the iSeq 100 System.

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iSeq 100 i1 Reagent v2 (300-cycle) 4 pack

20031374

Includes four i1 flow cells and four pre-filled cartridges containing clustering and sequencing reagents to support 4 × 300-cycle runs on the iSeq 100 System.

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iSeq 100 i1 Reagent v2 (300-cycle) 8 pack

20040760

Includes eight i1 flow cells and eight pre-filled cartridges containing clustering and sequencing reagents to support 8 × 300-cycle runs on the iSeq 100 System.

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Accessory products (3)

iSeq 100 Spare Air Filter

20023928

Includes air filter for iSeq 100 Sequencing System. Recommend replacing every six months.

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iSeq 100 Spare Drip Tray Pad

20023927

Includes drip tray pad for lining the drip tray and absorbing any leaked fluids.

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iSeq 100 System Test Kit

20024141

Includes reusable test cartridge and flow cell for use in validating proper iSeq 100 System performance. Replaces the reusable test components shipped with the instrument when they expire after 5 years or exceed 130 uses.

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Services (2)

iSeq 100 Installation and Operational Qualification

20023611

Verifies that the iSeq 100 Sequencing System has been delivered, installed, and is operating as intended according to manufacturer specifications. Appropriate documentation is provided.

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iSeq 100 Installation, Operational, and Performance Qualification

20023612

Verifies that the iSeq 100 Sequencing System has been delivered, installed, and is operating and performing as intended according to manufacturer specifications. Appropriate documentation is provided.

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Required products

iSeq 100 i1 Reagents are required to use the iSeq 100 System. These reagents include a flow cell and all necessary sequencing reagents integrated into a single cartridge.

The following are optional accessories designed to maintain the iSeq 100 System:

  • The iSeq 100 System Air Filter is necessary for peak performance of the iSeq 100 System and needs to be replaced every six months

  • The iSeq 100 System Drip Tray Pads are for lining the drip tray and absorbing any leaked fluids

  • The iSeq 100 System Test Kit includes reusable test cartridge and flow cell for use in validating proper iSeq 100 System performance. The test kit replaces the reusable test components shipped with the instrument after they expire

/ Results

FAQs

The iSeq 100 System supports read lengths up to 2 × 150 bp.

The iSeq 100 System flow cell can generate 1.2 Gb per sequencing run. For detailed performance parameters, see specifications for the iSeq 100 Sequencing System.

The number of libraries that can be sequenced at one time depends on the desired coverage for 4M reads. Using supported Illumina kits, up to 384 libraries can be multiplexed. For coverage considerations, see the sequencing coverage calculator.

Yes, iSeq 100 i1 Reagents include all consumables to support both single-read and paired-end sequencing. During run setup, you can configure the run to generate either single-read or paired-end data.

/ Results

NovaSeq X Series ordering

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MiSeq Reagent Kit v3

Optimized reagent kit that provides increased cluster density and read length, improving sequencing quality scores compared to earlier versions.

TruSight Oncology 500

Enable CGP with a large pan-cancer panel covering all major variant classes plus gene signatures (TMB, MSI, and HRD) from FFPE tissue.

Illumina DNA Prep

A fast, integrated workflow for preparing libraries for use in a wide range of sequencing applications.

COVIDSeq Assay (96 samples)

This low- to mid-throughput NGS assay enables labs of any size to identify and track the emergence and prevalence of novel SARS-CoV-2 variants.

Illumina Microbial Amplicon Prep

A flexible and streamlined NGS library prep kit that enables various public health surveillance and microbial research applications.

Nextera DNA Indexes

Nextera DNA Indexes enable preparation of high-quality libraries that can be multiplexed for increased throughput.

TruSight One Sequencing Panels

Comprehensive sequencing research panels targeting disease-associated regions of the exome with high analytical sensitivity and specificity.

Speak with a specialist

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