Targeted RNA expression panel investigating 395 genes involved in tumor-immune system interactions.
Assay time
Hands-on time
Input quantity
The AmpliSeq for Illumina Immune Response Panel is a targeted resequencing research assay for quantitating expression of cancer biomarkers in 395 genes involved in tumor–immune system interactions.
The Immune Response Panel is part of an integrated RNA-to-annotated variant solution that includes AmpliSeq for Illumina polymerase chain reaction (PCR)-based library preparation, Illumina sequencing by synthesis (SBS) next-generation sequencing (NGS) technology, and automated analysis.
This panel detects biomarkers associated with different leukocyte subsets, antigen presentation, checkpoint pathways, and tumor progression. The ready-to-use panel saves you the time and effort of identifying targets, designing primers, and optimizing panels.
| Assay time | 6 hours (library prep only; doesn't include library quantification, normalization, or pooling time) |
|---|---|
| Content specifications | 395 genes associated with immune response |
| Hands-on time | < 1.5 hr |
| Input quantity | 1–100 ng (10 ng recommended per pool) |
| Instruments | MiSeq System, NextSeq 550 System, NextSeq 2000 System, NextSeq 1000 System, MiSeqDx in Research Mode, MiniSeq System |
| Method | Amplicon sequencing, Targeted RNA sequencing |
| Multiplexing | 96 dual index combinations |
| Nucleic acid type | RNA |
| Number of amplicons | 398 total (1 pool) |
| Specialized sample types | FFPE tissue |
| Species category | Human |
| Technology | Sequencing |
| Variant class | Transcript variants |
The AmpliSeq for Illumina Immune Response Panel workflow requires the Immune Response Panel, Library PLUS kit, and index adapter. Also, the cDNA synthesis kit is required to convert total RNA to cDNA.
AmpliSeq for Illumina Immune Response Panel
| Instrument | Recommended number of samples | Read length |
|---|---|---|
| iSeq 100 System | 4 samples per run (assumes 1 million reads per sample) |
2 × 150 bp |
| MiniSeq System | Samples per run: mid output: 8, high output: 24 (assumes 1 million reads per sample) |
2 × 150 bp |
| MiSeq System | Samples per run (by reagent kit version): v2 micro: 4, v2: 15, v3: 24 (assumes 1 million reads per sample) |
2 × 150 bp |
With targeted resequencing, a subset of genes or a genomic region is isolated and sequenced, which can conserve lab resources.
Illumina NGS and microarray technologies for cancer research are helping drive the revolution in cancer genomics.
Gain insight into the immune repertoire, disease mechanisms, and the functional impact of immune-related genetic variation with NGS and array solutions.
AmpliSeq™ Immune Response Panel for Illumina®
20019169
Sufficient for 24 reactions when paired with AmpliSeq Library PLUS for Illumina. Purchase library prep, index adapters, and accessory products separately.
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AmpliSeq™ Library PLUS (24 Reactions) for Illumina®
20019101
Includes reagents for preparing 24 libraries. Purchase AmpliSeq for Illumina panel and index adapters separately.
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AmpliSeq™ Library PLUS (96 Reactions) for Illumina®
20019102
Includes reagents for preparing 96 libraries. Purchase AmpliSeq for Illumina panel and index adapters separately.
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AmpliSeq™ Library PLUS (384 Reactions) for Illumina®
20019103
Includes reagents for preparing 384 libraries. Purchase AmpliSeq for Illumina panel and index adapters separately.
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AmpliSeq™ UD Indexes for Illumina® (24 Indexes, 24 Samples)
20019104
Includes 24 indexes sufficient for labeling 24 samples. Purchase library prep and enrichment reagents and probe panels separately.
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AmpliSeq™ CD Indexes Set A for Illumina®
20019105
Includes 96 indexes sufficient for labeling 96 samples. Purchase library prep and enrichment reagents and probe panels separately.
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AmpliSeq™ CD Indexes Set B for Illumina®
20019106
Includes 96 indexes sufficient for labeling 96 samples. Purchase library prep and enrichment reagents and probe panels separately.
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AmpliSeq™ CD Indexes Set C for Illumina®
20019107
Includes 96 indexes sufficient for labeling 96 samples. Purchase library prep and enrichment reagents and probe panels separately.
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AmpliSeq™ CD Indexes Set D for Illumina®
20019167
Includes 96, 8 bp indexes sufficient for labeling 96 samples. Purchase library prep reagents and probe panels separately.
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AmpliSeq™ CD Indexes Set A-D for Illumina® (384 Indexes, 384 Samples)
20031676
Includes four sets of AmpliSeq CD Indexes for Illumina, Set A, Set B, Set C and Set D. Each set contains 96, 8 bp indexes sufficient for labeling 96 samples. Purchase library prep and probe panels separately.
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Ampliseq™ cDNA Synthesis for Illumina®
20022654
Includes reaction mix and enzyme blend to convert total RNA to cDNA when working with AmpliSeq for Illumina RNA Panels (eg, AmpliSeq for Illumina Immune Response, Immune Repertoire Plus, TCR beta Panel, Myeloid, Transcriptome Human Gene Expression, Focus, and Comprehensive Panel v3 as well as Custom or Community RNA Panels). Number of reactions vary by panel (eg, 100 reactions per kit for AmpliSeq for Illumina Immune Response, Focus, Comprehensive Panel v3, and Custom panels and 200 reactions per kit for AmpliSeq for Illumina Transcriptome Human Gene Expression, Myeloid, Immune Repertoire Plus, and TCR beta Panels).
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AmpliSeq™ for Illumina® Direct FFPE DNA
20023378
Includes 24 reactions to prepare DNA from unstained, slide-mounted FFPE tissues for library construction using AmpliSeq for Illumina protocol, without the need for deparaffinization or DNA purification.
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AmpliSeq™ Library Equalizer for Illumina®
20019171
Includes beads and reagents for library normalization when preparing AmpliSeq for Illumina libraries for sequencing.
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