A 58-gene pan-cancer panel with combined DNA content, utilizing the single-tube SLIMamp workflow for fast library prep and turnaround time.
Assay time
Hands-on time
Input quantity
The oncoReveal Myeloid Panel is a DNA NGS sequencing panel using proprietary Stem-Loop Inhibition-Mediated amplification (SLIMamp) technology, a tiled amplicon-based library prep chemistry for efficient single-tube target enrichment.
| Assay time | 48 hr from sample to report |
|---|---|
| Cancer type | Hematologic |
| Description | 58-gene amplicon based DNA panel, simple single tube workflow |
| Hands-on time | ~3 hr |
| Input quantity | 10–80 ng DNA (20 ng recommended) |
| Instruments | MiSeq System, NextSeq 550 System, MiSeqDx in Research Mode, NextSeq 550Dx in Research Mode, MiSeq i100 System, MiSeq i100 Plus System |
| Mechanism of action | Multiplex PCR using tiled amplicon |
| Method | Targeted DNA sequencing, Amplicon sequencing |
| Nucleic acid type | DNA |
| Number of amplicons | 1 pool, 766 amplicons |
| Number of reactions | 24 reactions |
| On-target reads | 99.6% ± 0.2% |
| Specialized sample types | Blood, Low-input samples |
| Species category | Human |
| Target insert size | Average: 215 bp |
| Technology | Sequencing |
| Uniformity | 96.8% ±1.0% |
| Variant class | Single nucleotide variants (SNVs), Insertions-deletions (indels), Copy number variants (CNVs) |
Pillar oncoReveal panels require the use of a Pillar index kit. Pick your preferred number of reactions.
Pillar oncoReveal Myeloid Panel
| Pillar oncoReveal Myeloid Panel | AmpliSeq for Illumina Myeloid Panel | Pillar oncoReveal Essential MPN Panel | |
|---|---|---|---|
| Assay time | 48 hr from sample to report | 5.5 – 7.5 hours (library prep only; doesn't include library quantification, normalization, or pooling time) | 48 hr from sample to report |
| Cancer type | Hematologic | Hematologic | Hematologic |
| Description | 58-gene amplicon based DNA panel, simple single tube workflow | Targeted panel to study 40 DNA genes, 29 RNA fusion driver genes, and 5 gene expression levels associated with myeloid cancers. | A targeted gene panel focused on mutations affecting MPL, JAK2, and CALR, simple single tube workflow |
| Hands-on time | ~3 hr | < 1.5 hr | ~3 hr |
| Input quantity | 10–80 ng DNA (20 ng recommended) |
20 ng high-quality DNA; 10 ng high-quality RNA (10 ng recommended per pool) |
10–80 ng DNA (20 ng recommended) |
| Instruments | MiSeq System, NextSeq 550 System, MiSeqDx in Research Mode, NextSeq 550Dx in Research Mode, MiSeq i100 System, MiSeq i100 Plus System | MiSeq System, MiniSeq System | MiSeq System, NextSeq 550 System, MiSeqDx in Research Mode, NextSeq 550Dx in Research Mode, MiSeq i100 System, MiSeq i100 Plus System |
| Mechanism of action | Multiplex PCR using tiled amplicon | Multiplex PCR using tiled amplicon | |
| Method | Targeted DNA sequencing, Amplicon sequencing | Targeted DNA sequencing, Amplicon sequencing, Targeted RNA sequencing | Targeted DNA sequencing, Amplicon sequencing |
| Nucleic acid type | DNA | DNA, RNA | DNA |
| Number of amplicons | 1 pool, 766 amplicons | 3 genes, 7 amplicons | |
| Number of reactions | 24 reactions | 48 reactions per kit | |
| On-target reads | 99.6% ± 0.2% | 99.9% ± 0.0% | |
| Specialized sample types | Blood, Low-input samples | Blood, Not FFPE-compatible, Bone marrow | Blood, Low-input samples |
| Species category | Human | Human | Human |
| Target insert size | Average: 215 bp | Average: 167bp | |
| Technology | Sequencing | Sequencing | Sequencing |
| Uniformity | 96.8% ±1.0% | 100% | |
| Variant class | Single nucleotide variants (SNVs), Insertions-deletions (indels), Copy number variants (CNVs) | Single nucleotide polymorphisms (SNPs), Gene fusions, Somatic variants, Insertions-deletions (indels) | Somatic variants, Single nucleotide variants (SNVs), Insertions-deletions (indels) |
Pillar oncoReveal Myeloid Panel interrogates 58 genes from DNA.
Pillar oncoReveal Myeloid Panel specifications for DNA variant interrogation of 58 genes from blood.
oncoReveal™ Myeloid Panel
HDA-MY-1001-24
oncoReveal™ Myeloid Panel
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Indices PI501-8, PI701-4 (32 combinations, 96 reactions)
IDX-PI-1001-96
Indices PI501-8, PI701-4 (32 combinations, 96 reactions)
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Kit D, indices PI501-8, PI701-12
IDX-PI-1004-192
Kit D, indices PI501-8, PI701-12
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Reagents for the MiSeq i100 Series feature easy-to-use cartridges and multiple flow cell configurations for flexible sequencing options.
A robust assay targeting key mutations in MPL, JAK2, and CALR genes, utilizing single-tube SLIMamp workflow for fast library prep and turnaround time.
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