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Pillar oncoReveal Essential MPN Panel

A robust assay targeting key mutations in MPL, JAK2, and CALR genes, utilizing single-tube SLIMamp workflow for fast library prep and turnaround time.

48 hr from sample to report

Assay time

~3 hr

Hands-on time

10–80 ng DNA (20 ng recommended)

Input quantity

See full details in the specifications table

Overview

The Pillar oncoReveal Essential MPN Panel is a robust assay that simultaneously tests for key mutations within the MPL, JAK2, and CALR genes. The assay uses proprietary Stem Loop Inhibition-Mediated amplification (SLIMamp) technology, a tiled amplicon-based library prep chemistry for efficient single-tube target enrichment.

  • Maintain control of samples and results with single-tube, tiled amplification that can be performed in-house by any NGS lab

  • Achieve variant detection as low as 1% VAF without UIDs, even with limited DNA input or poor sample quality

  • Improve lab efficiency and reduce “no calls,” repeat testing, and difficult interpretation decisions

  • Less than 8 hours from DNA to sequencer

Specifications

Assay time 48 hr from sample to report
Cancer type Hematologic
Description A targeted gene panel focused on mutations affecting MPL, JAK2, and CALR, simple single tube workflow
Hands-on time ~3 hr
Input quantity 10–80 ng DNA (20 ng recommended)
Instruments MiSeq System, NextSeq 550 System, MiSeqDx in Research Mode, NextSeq 550Dx in Research Mode, MiSeq i100 System, MiSeq i100 Plus System
Mechanism of action Multiplex PCR using tiled amplicon
Method Targeted DNA sequencing, Amplicon sequencing
Nucleic acid type DNA
Number of amplicons 3 genes, 7 amplicons
Number of reactions 48 reactions per kit
On-target reads 99.9% ± 0.0%
Specialized sample types Blood, Low-input samples
Species category Human
Target insert size Average: 167bp
Technology Sequencing
Uniformity 100%
Variant class Somatic variants, Single nucleotide variants (SNVs), Insertions-deletions (indels)

Required Products

Pillar oncoReveal panels require the use of a Pillar index kit. Pick your preferred number of reactions.

/ Results

Applications

Example workflow

1
Prep

Pillar oncoReveal Essential MPN Panel

2
Sequence
3
Analyze

Documentation

Product literature
Support documentation

Compare

Pillar oncoReveal Essential MPN Panel AmpliSeq for Illumina Myeloid Panel Pillar oncoReveal Myeloid Panel
Assay time 48 hr from sample to report 5.5 – 7.5 hours (library prep only; doesn't include library quantification, normalization, or pooling time) 48 hr from sample to report
Cancer type Hematologic Hematologic Hematologic
Description A targeted gene panel focused on mutations affecting MPL, JAK2, and CALR, simple single tube workflow Targeted panel to study 40 DNA genes, 29 RNA fusion driver genes, and 5 gene expression levels associated with myeloid cancers. 58-gene amplicon based DNA panel, simple single tube workflow
Hands-on time ~3 hr < 1.5 hr ~3 hr
Input quantity 10–80 ng DNA (20 ng recommended) 20 ng high-quality DNA; 10 ng high-quality RNA
(10 ng recommended per pool) 		10–80 ng DNA (20 ng recommended)
Instruments MiSeq System, NextSeq 550 System, MiSeqDx in Research Mode, NextSeq 550Dx in Research Mode, MiSeq i100 System, MiSeq i100 Plus System MiSeq System, MiniSeq System MiSeq System, NextSeq 550 System, MiSeqDx in Research Mode, NextSeq 550Dx in Research Mode, MiSeq i100 System, MiSeq i100 Plus System
Mechanism of action Multiplex PCR using tiled amplicon Multiplex PCR using tiled amplicon
Method Targeted DNA sequencing, Amplicon sequencing Targeted DNA sequencing, Amplicon sequencing, Targeted RNA sequencing Targeted DNA sequencing, Amplicon sequencing
Nucleic acid type DNA DNA, RNA DNA
Number of amplicons 3 genes, 7 amplicons 1 pool, 766 amplicons
Number of reactions 48 reactions per kit 24 reactions
On-target reads 99.9% ± 0.0% 99.6% ± 0.2%
Specialized sample types Blood, Low-input samples Blood, Not FFPE-compatible, Bone marrow Blood, Low-input samples
Species category Human Human Human
Target insert size Average: 167bp Average: 215 bp
Technology Sequencing Sequencing Sequencing
Uniformity 100% 96.8% ±1.0%
Variant class Somatic variants, Single nucleotide variants (SNVs), Insertions-deletions (indels) Single nucleotide polymorphisms (SNPs), Gene fusions, Somatic variants, Insertions-deletions (indels) Single nucleotide variants (SNVs), Insertions-deletions (indels), Copy number variants (CNVs)

Figures

Gene List

Pillar oncoReveal Essential MPN Panel interrogates 6 exons across the MPL, JAK2, and CALR genes.

Panel Specifications

Pillar oncoReveal Essential MPN Panel specifications for DNA variant interrogation of MPL, JAK2, and CALR from blood.

oncoReveal™ Essential MPN Panel

HDA-MY-1002-48

oncoReveal™ Essential MPN Panel

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Indices PI501-8, PI701-4 (32 combinations, 96 reactions)

IDX-PI-1001-96

Indices PI501-8, PI701-4 (32 combinations, 96 reactions)

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Kit D, indices PI501-8, PI701-12

IDX-PI-1004-192

Kit D, indices PI501-8, PI701-12

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