TruSight RNA Fusion Panel

Comprehensive gene fusion detection covering 507 genes from FFPE and other cancer research samples.

2.5 days

Assay time

11 hr

Hands-on time

10 ng total RNA, 20–100 ng FFPE RNA

Input quantity

See full details in the specifications table

Overview

The TruSight RNA Fusion Panel covers 507 fusion-associated genes related to cancer in a single NGS assay for research use, providing results from RNA in four days. 

  • Industry-guided content for comprehensive detection of common and novel fusions

  • Optimized RNA sequencing for all sample types, including formalin-fixed, paraffin-embedded (FFPE) tissue 

  • On-instrument software that displays fusion calls 

A comprehensive view of cancer-related fusion genes

The TruSight RNA Fusion Panel covers hundreds of genes in a single assay, increasing the possibility of fusion detection in the first round of testing.

Simple, streamlined workflow

A simple integrated workflow includes library prep, sequencing, and data analysis and can be scaled according to the number of samples. RNA samples can be multiplexed and sequenced on a benchtop system to maximize lab budgets.

On-instrument software

On-instrument fusion calling provides NGS-based fusion detection to labs without additional bioinformatics support. The analysis provides a list of detected fusions, relevant disease associations (as identified by the Mitelman Database), and evidence of fusion-supporting reads. 


Specifications


Required products

Requires purchase of both Set A and Set B kitted reagents, each containing 12 unique indexes.  

/ Results

Applications

TruSight RNA Fusion Panel enables gene fusion detection studies with known and novel partners in 507 fusion-associated genes in multiple cancer types using blood, bone marrow, or FFPE samples. 

Example workflow

1
Prep
TruSight RNA Fusion Panel

Project recommendations

Instrument Recommended number of samples Read length
MiniSeq System

Samples per run: mid output: 2-3, high output: 8 (based on 3 million reads per sample)

2 × 76 bp (max recommended)

MiSeq System

8 samples per run with v3 reagents (based on 3 million reads per sample)

2 × 76 bp (max recommended)

NextSeq 550 System

24 samples per run (mid output; based on 3 million reads per sample)

2 × 76 bp (max recommended)


Related applications and methods

Compare

TruSight RNA Fusion Panel TruSight Tumor 170 AmpliSeq for Illumina Focus Panel AmpliSeq for Illumina Comprehensive Panel v3
Assay time 2.5 days ~2 days 5-6 hr (library prep only; doesn't include library quantification, normalization, or pooling time) 5-6 hr (library prep only; doesn't include library quantification, normalization, or pooling time)
Automation details Explore available automation methods Explore available automation methods
Cancer type Pan-Cancer, Hematologic, Solid Tumor Solid Tumor Solid Tumor Solid Tumor
Description

Provides a cost-effective NGS solution for cancer research, offering deep coverage of 507 genes implicated in multiple cancer types, including solid tumors, sarcomas, and hematological malignancies. 

Perform comprehensive somatic variant detection research in solid tumors using variant calling information from both DNA and RNA. Somatic analysis research on 52 genes associated with solid tumor cancers. Somatic analysis research on hotspot and full-length targets of genes* associated with solid tumor cancers.
Hands-on time 11 hr ~10.5 hr < 1.5 hr <1.5 hr
Input quantity 10 ng total RNA, 20–100 ng FFPE RNA 40 ng DNA and/or RNA 1–100 ng (10 ng recommended per pool) 1–100 ng (10 ng recommended per pool)
Instruments MiSeq System, NextSeq 550 System, MiSeqDx in Research Mode, MiniSeq System, NextSeq 500 System NextSeq 550 System, NextSeq 500 System, HiSeq 2500 System MiSeq System, iSeq 100 System, MiSeqDx in Research Mode, MiniSeq System NextSeq 550 System, NextSeq 2000 System, NextSeq 1000 System, NextSeq 550Dx in Research Mode
Method Targeted RNA sequencing, Target enrichment Targeted DNA sequencing, Targeted RNA sequencing, Target enrichment Targeted DNA sequencing, Amplicon sequencing, Targeted RNA sequencing Targeted DNA sequencing, Amplicon sequencing, Targeted RNA sequencing
Nucleic acid type RNA DNA, RNA DNA, RNA DNA, RNA
Specialized sample types Low-Input Samples, FFPE Tissue Low-Input Samples, FFPE Tissue FFPE Tissue FFPE Tissue
Species category Human Human Human Human
Technology Sequencing Sequencing Sequencing Sequencing
Variant class Gene fusions Gene fusions, Somatic variants, Structural variants, Single nucleotide variants (SNVs), Insertions-deletions (indels), Copy number variants (CNVs) Single nucleotide polymorphisms (SNPs), Gene fusions, Somatic variants, Insertions-deletions (indels), Copy number variants (CNVs) Single nucleotide polymorphisms (SNPs), Gene fusions, Somatic variants, Insertions-deletions (indels), Copy number variants (CNVs)

Selection tools:

Figures

TruSight RNA Fusion capture chemistry

The TruSight RNA Fusion Panel provides a simple, streamlined method for isolating targeted regions of interest from total RNA.

Concordance of TruSight RNA Fusion Detection with FISH and RT-PCR

Fusion detection in FFPE samples using the TruSight RNA Fusion Panel

The Trusight RNA Fusion Panel is optimized for high performance from both high- and low-quality RNA sample types. Library prep requires as little as 10 ng total RNA, or 20 ng FFPE RNA. This low input requirement makes this panel ideal for reliable targeted analysis of limited quantity samples.

TruSight RNA Fusion Panel Set A (48 samples)

RS-304-1002

Kitted reagents for RNA library prep and enrichment of 48 total RNA samples (including FFPE) for sequencing. Each kit contains 12 unique indexes; 2, 4, 5, 6, 7, 12, 13, 14, 15, 16, 18, and 19.

Sign in to add to cart or see pricing.

List Price:

Discounts:

TruSight RNA Fusion Panel Set B (48 samples)

RS-304-1003

Kitted reagents for RNA library prep and enrichment of 48 total RNA samples (including FFPE) for sequencing. Each kit contains 12 unique indexes; 1, 3, 8, 9,10, 11, 20, 21, 22, 23, 25, and 27.

Sign in to add to cart or see pricing.

List Price:

Discounts:

Trusight RNA Fusion Oligo Panel

20046101

Enrichment oligos only. Library prep and index adapter reagents need to be ordered separately

Sign in to add to cart or see pricing.

Request pricing

List Price:

Discounts:

Showing of

Selection summary

Product

Qty

Unit Price

FAQs

A high-confidence fusion call means that a fusion meets the threshold filters based on scores from calculated values of split read scores, paired read scores, break-end homology, and other features.

A low- confidence fusion call means that a fusion is listed as recurrent in the Mitelman database but does not pass or meet the minimum threshold score. 

Learn more in Local Run Manager RNA Fusion Analysis Module Workflow Guide

The Fusion Score reflects the confidence in the fusion call, where 0 is low and 1 is high. Scores > 0.6 are reported as high- confidence fusion calls.

This assay is being automated by some of our automation providers. If you are interested in automating this workflow, contact your account manager.

Chromosomal translocations resulting in overexpression or deletion of a transcript can be reflected in gene expression levels but would not create a fusion gene. The Local Run Manager RNA Fusion module is not designed for detection of gene expression changes. To detect these changes, the RNA-Seq Alignment App in BaseSpace Sequence Hub is recommended. In addition, it is recommended to confirm these findings in DNA. 

/ Results

Frequently purchased

Order the NovaSeq X Series

Advanced chemistry, optics, and informatics combine to deliver exceptional speed and data quality, outstanding throughput and scalability.

MiSeq Reagent Kit v3

Optimized reagent kit that provides increased cluster density and read length, improving sequencing quality scores compared to earlier versions.

TruSight Oncology 500

Enable CGP with a large pan-cancer panel covering all major variant classes plus gene signatures (TMB, MSI, and HRD) from FFPE tissue.

Illumina DNA Prep

A fast, integrated workflow for preparing libraries for use in a wide range of sequencing applications.

Speak with a specialist

Reach out for information about our products and services, or get answers to questions about our technology.