NovaSeq X Applications & Methods

Applications & Methods

Unimaginable experiments, made unbelievably cost-efficient

The NovaSeq X Series offers vast application breadth, enabling data-intensive methods at production scale

Key Applications and Methods

The unmatched combination of production-scale throughput and cost enables deeper sequencing of larger sample cohorts. The NovaSeq X Plus system delivers up to 16 Tb of output, allowing sequencing of 128 human genomes at 30× coverage or 96 human genomes at 40× coverage per dual 25B flow cell run.

Illumina Complete Long Reads enables generation of both long- and short-reads on the same instrument for more scalable, comprehensive whole-genome sequencing.

three to five hours
Prep
Illumina DNA PCR-Free Prep

A high-performing, fast, and integrated workflow for applications such as whole-genome sequencing or tumor-normal sequencing.

Illumina Complete Long Read Prep

Illumina Complete Long Read Prep, Human, is designed for human whole-genome sequencing (WGS).

less than or equal to 44 hours
Sequence
NovaSeq X Series Reagent Kits

The power of XLEAP-SBS chemistry combined with flexible, efficient, and sustainable workflows for transformational genomics at scale.

The 25B flow cell unlocks access to the highest throughput sequencing run on an Illumina sequencer. With 8 individually addressable lanes, and 2.5x greater output per run than the NovaSeq 6000 System, the 25B unlocks unprecedented sequencing scale and significant multiplexing to enable the deepest, broadest, and most cost-effective sequencing projects.

less than 25 minutes
Analyze
DRAGEN Germline Pipeline

Full genome analysis with increased accuracy for variant calling, including repeat expansion detection and genotyping.

DRAGEN Illumina Complete Long Read WGS

Generates our most accurate and comprehensive whole human genome data using DRAGEN and Illumina Complete Long Reads.

Representing less than 2% of the genome, WES is a cost-effective alternative to WGS. The NovaSeq X Plus system offers sequencing of ~1500 exomes in a single run using a dual 25B flow cell run.

six hours
Prep
Illumina DNA Prep with Enrichment

This kit uses a fast, user-friendly workflow. On-bead tagmentation chemistry is combined with a simplified, single hybridization protocol to reduce total workflow time.

about twenty-five hours
Sequence
NovaSeq X Series Reagent Kits

The power of XLEAP-SBS chemistry combined with flexible, efficient, and sustainable workflows for transformational genomics at scale.

less than eight minutes
Analyze
DRAGEN Enrichment Pipeline

Exome analysis for variant detection, including a full suite of enrichment metrics and reporting.

Analyze coding plus multiple forms of noncoding RNA for a comprehensive picture of the transcriptome. With the NovaSeq X Plus system, you can sequence over 1000 transcriptomes in a single run using a dual 25B flow cell run.

about seven hours
Prep
Illumina Stranded Total RNA Prep

Rapid library preparation from a broad range of sample types for studying the coding and non-coding transcriptome with unparalleled study flexibility.

about twenty-five hours
Sequence
NovaSeq X Series Reagent Kits

The power of XLEAP-SBS chemistry combined with flexible, efficient, and sustainable workflows for transformational genomics at scale.

less than fifteen minutes
Analyze
DRAGEN RNA Pipeline

Performs alignment, quantification, and fusion detection.

Brochure cover: Unlock the next wave of genomic discovery
Unlock the next wave of genomic discovery

Increased discovery power will come from larger studies, deeper sequencing to identify rare genetic events, and broader sequencing methods and multiomics for a more comprehensive view of cellular activity. Learn how you can perform broader, deeper sequencing and take on projects that were previously out of reach with the NovaSeq X Series.

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More Applications and Methods

Methylation Sequencing

Enhance epigenetic studies with high-coverage density and flexibility enabled by sequencing-based DNA methylation analysis.

Metagenomic Sequencing

Sequence thousands of organisms in parallel. Identify unculturable or low-abundance microbes, or evaluate microbial diversity.

mRNA Sequencing

Select and sequence polyadenylated transcripts for gene expression profiling studies.

High-Intensity Sequencing

Improve statistical power, get multidimensional insights, and increase analytical resolution with cost-efficient high-throughput sequencing workflows.

Tumor-Normal Sequencing

Genome-wide comparison of tumor vs matched normal DNA to inform analysis of oncogenes, tumor suppressors, and other risk factors.

Single-Cell RNA Seq

Assess the individual contributions of single cells in complex tissues by profiling the transcriptome.

ATAC-Seq

The assay for transposase-accessible chromatin with sequencing (ATAC-Seq) is a popular method for determining chromatin accessibility across the genome.

ChIP-Seq

Chromatin immunoprecipitation sequencing (ChIP-Seq) is a powerful method for analyzing DNA-protein interactions and performing genome-wide surveys of gene regulation.

De Novo Sequencing

De novo sequencing refers to sequencing a novel genome with no reference sequence available. NGS enables fast, accurate characterization of any species.

Target Enrichment

Capture genomic regions of interest via hybridization to target-specific biotinylated probes.

Long Read Sequencing

A highly accurate approach that can help resolve challenging regions of the genome such as complex structural variants and highly repetitive elements.