This sequencing panel offers a quick, accurate, and cost-effective method to research the SARS-CoV-2 virus.
This research panel contains 247 amplicons in 2 pools targeting the SARS-CoV-2 genome. The panel includes >99% coverage of the Coronavirus genome (~30kb) and covers all potential serotypes.
AmpliSeq for Illumina Community Panels are made-to-order. We provide the designs for your convenience; however, they do not have associated performance metrics. For predesigned and tested content, view AmpliSeq for Illumina Ready-to-Use Panels.
AmpliSeq for Illumina Community Panels contain content selected and designed with input from leading disease researchers. You can order this community panel as is, or customize the panel to meet your needs, using Illumina Concierge to add or remove genes and amplicons.
| Input quantity | 1–100 ng (10 ng recommended per pool) |
|---|---|
| Method | Amplicon sequencing |
| Nucleic acid type | RNA |
| Number of amplicons | 247 total. 2 pools. (Pool 1: 125 amplicons. Pool 2: 122 amplicons). |
| Species category | Virus |
| Species details | SARS-CoV-2 (SARS-CoV-2_ MN908947v3) |
| Technology | Sequencing |
In addition to the community panel, you will also need an AmpliSeq Library PLUS kit and index adapters.
| AmpliSeq for Illumina SARS-CoV-2 Research Panel | Illumina Respiratory Virus Enrichment Kit | Respiratory Pathogen ID/AMR Enrichment Panel Kit | COVIDSeq Test (RUO Version) | ||
|---|---|---|---|---|---|
| Input quantity | 1–100 ng (10 ng recommended per pool) | 10-100 ng | Based on volume (not concentration dependent) | ||
| Method | Amplicon sequencing | Targeted DNA sequencing, Targeted RNA sequencing, Target enrichment | Targeted DNA sequencing, Targeted RNA sequencing, Target enrichment | Amplicon sequencing, Targeted RNA sequencing | |
| Nucleic acid type | RNA | DNA, RNA | DNA, RNA | RNA | |
| Number of amplicons | 247 total. 2 pools. (Pool 1: 125 amplicons. Pool 2: 122 amplicons). | ||||
| Species category | Virus | Human, Virus | Fungal, Virus, Bacteria | Human, Virus | |
| Species details | SARS-CoV-2 (SARS-CoV-2_ MN908947v3) | Detects common respiratory viruses, including recent influenza virus strains and SARS-CoV-2. The panel includes probes to human targets as a quality feature, and therefore detects those human targets in every sample assayed. | Detects respiratory pathogens (180+ bacteria, 50+ fungi, and 40+ viruses, including SARS-CoV-2) and antimicrobial resistance alleles (1200+). | SARS-CoV-2 (SARS-CoV-2_ MN908947v3) | |
| Technology | Sequencing | Sequencing | Sequencing | Sequencing |
AmpliSeq™ SARS-CoV-2 Research Panel for Illumina®
20020496
Targeted custom amplicon panel optimized for specific targets or genomic content of interest. Library PLUS and index adapters sold separately.
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The AmpliSeq for Illumina SARS-CoV-2 Community Panel, in combination with AmpliSeq for Illumina library prep, index, and accessories, is a targeted RNA/cDNA amplicon assay for epidemiological research of the SARS-CoV-2 virus (Research Use Only). The panel is a 2-pool design, containing 247 amplicons/primer pairs (242 unique amplicons (Pool 1: 125 amplicon, Pool 2: 122 amplicons): 237 viral specific SARS-CoV-2 targets and 5 human gene expression controls) ranging from 125-275 bps in length that covers >99% of the viral genome and all potential serotypes of the virus.
The panel is intended for research use only (RUO) applications, and not for use in diagnostic procedures. No claim or representation is intended to provide information to indicate the panel may be used in diagnosis, prevention, or management of an infection.
We recommend customers evaluate commercially available extraction kits capable of isolating viral RNA. The QIAGEN QIAmp Viral RNA Mini Kit (PN 52904) has been demonstrated to be compatible for use in next-generation sequencing library preparation and other testing methods internally. In the event of a back order, any commercially available extraction kit from other companies should be compatible as well.
See the following resources for additional information:
Library preparation for the AmpliSeq for Illumina SARS-CoV-2 Community Panel follows the AmpliSeq for Illumina Custom and Community Panels Reference Guide: Chapter 3 Protocol for RNA Panels (https://support.illumina.com/downloads/ampliseq-for-illumina-custom-and-community-panels-reference-guide-1000000036408.html).
In “Amplify cDNA Targets”, proceed with the following:
Library preparation using the AmpliSeq Library PLUS for Illumina is compatible with all AmpliSeq CD or UD Index Sets for Illumina.
Read depth: 250,000 reads per sample.
Read length: 2 x 151 bp.
Platforms: AmpliSeq for Illumina libraries are compatible with iSeq 100, MiSeq, MiniSeq, and NextSeq systems.
After adding the panel to your MyIllumina cart and checking out, you will receive your panel order. The panel should state the solution ID: IAACY126664577_100.
Depending on sample throughput, the customer also needs to order the following:
1 order contains enough oligos to prepare 2250 samples.
1 order = $2223 USD is sufficient for preparing 2250 samples (~$9/amplicon). This does not include AmpliSeq Library PLUS for Illumina, Ampliseq cDNA Synthesis for Illumina, or AmpliSeq CD or UD Indexes for Illumina.
The BaseSpace Sequence Hub DNA Amplicon or DRAGEN Metagenomics Pipeline apps can be used for data analysis.
For additional questions please contact techsupport@illumina.com
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