AmpliSeq for Illumina Immune Repertoire Plus, TCR beta Panel

Targeted RNA research panel to investigate T cell diversity and clonal expansion by sequencing T cell receptor beta chain rearrangements. Read More...
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Panel

AmpliSeq™ for Illumina® Immune Repertoire Plus, TCR beta Panel

20024479

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Library Prep

AmpliSeq™ Library PLUS (24 Reactions) for Illumina®

20019101

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AmpliSeq™ Library PLUS (96 Reactions) for Illumina®

20019102

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AmpliSeq™ Library PLUS (384 Reactions) for Illumina®

20019103

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Index Adapters

AmpliSeq™ UD Indexes for Illumina® (24 Indexes, 24 Samples)

20019104

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AmpliSeq™ CD Indexes Set A for Illumina®

20019105

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AmpliSeq™ CD Indexes Set B for Illumina®

20019106

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AmpliSeq™ CD Indexes Set C for Illumina®

20019107

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AmpliSeq™ CD Indexes Set D for Illumina®

20019167

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AmpliSeq™ CD Indexes Set A-D for Illumina® (384 Indexes, 384 Samples)

20031676

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Accessory Products

Ampliseq™ cDNA Synthesis for Illumina®

20022654

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AmpliSeq™ Library Equalizer for Illumina®

20019171

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Product Highlights

AmpliSeq for Illumina Immune Repertoire Plus, TCR beta Panel is a highly multiplexed targeted resequencing panel to measure T cell diversity and clonal expansion by sequencing T cell receptor (TCR) beta chain rearrangements. Use a single pool of multiplex PCR primers, library reagents, and sample barcodes, to generate libraries from RNA extracted from whole blood, fresh-frozen tissue, or FACS-sorted cells. Key features include:

High-Value Content
  • Obtain comprehensive coverage of TCR beta chain sequences
Fast, Streamlined Workflow
  • Prepare sequencing-ready libraries in a single day from whole blood, fresh/frozen tissue, or fluorescence-activated cell sorting (FACS) cells
Low input requirement
  • Generate high-quality sequencing libraries from as little as 10 ng of input RNA

The Immune Repertoire Plus, TCR beta Panel is part of an integrated workflow that includes AmpliSeq for Illumina polymerase chain reaction (PCR)-based library preparation, Illumina sequencing by synthesis (SBS), NGS technology, and automated analysis.

This ready-to-use panel saves you the time and effort of identifying targets, designing primers, and optimizing panels.

Learn More About AmpliSeq for Illumina

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Specifications

Project Recommendations

Instrument Recommended Number of Samples Read Length
MiniSeq System Samples per run will depend on sample type. 2 × 150 bp
MiSeq Samples per run will depend on sample type. 2 x 300 bp
NextSeq 550 System Samples per run will depend on sample type. 2 × 150 bp

Product Comparison

AmpliSeq for Illumina Immune Repertoire Plus, TCR beta Panel AmpliSeq for Illumina TCR beta-SR Panel
Content Specifications RNA evaluation of TCRβ chain rearrangements, including CDR1, CDR2, and CDR3 (with up to 400 bp read-length amplicons) Sequences TCR beta chain rearrangements, with up to 80 bp read-length amplicons for characterizing CDR3.
Method Amplicon Sequencing, Targeted RNA Sequencing Amplicon Sequencing, Targeted DNA Sequencing, Targeted RNA Sequencing
Nucleic Acid Type RNA RNA, DNA
Specialized Sample Types Blood, FACS Cells, Fresh/Frozen Tissue Blood, FACS Cells, FFPE Tissue, Fresh/Frozen Tissue
Species Category Human Human

Supporting Data and Figures

 

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