AmpliSeq for Illumina Immune Repertoire Plus, TCR beta Panel is a highly multiplexed targeted resequencing panel to measure T cell diversity and clonal expansion by sequencing T cell receptor (TCR) beta chain rearrangements. Use a single pool of multiplex PCR primers, library reagents, and sample barcodes, to generate libraries from RNA extracted from whole blood, fresh-frozen tissue, or FACS-sorted cells. Key features include:
The Immune Repertoire Plus, TCR beta Panel is part of an integrated workflow that includes AmpliSeq for Illumina polymerase chain reaction (PCR)-based library preparation, Illumina sequencing by synthesis (SBS), NGS technology, and automated analysis.
This ready-to-use panel saves you the time and effort of identifying targets, designing primers, and optimizing panels.
Instrument | Recommended Number of Samples | Read Length |
---|---|---|
MiniSeq System | Samples per run will depend on sample type. | 2 × 150 bp |
MiSeq | Samples per run will depend on sample type. | 2 x 300 bp |
NextSeq 550 System | Samples per run will depend on sample type. | 2 × 150 bp |
AmpliSeq for Illumina Immune Repertoire Plus, TCR beta Panel | AmpliSeq for Illumina TCR beta-SR Panel | |
---|---|---|
Content Specifications | RNA evaluation of TCRβ chain rearrangements, including CDR1, CDR2, and CDR3 (with up to 400 bp read-length amplicons) | Sequences TCR beta chain rearrangements, with up to 80 bp read-length amplicons for characterizing CDR3. |
Method | Amplicon Sequencing, Targeted RNA Sequencing | Amplicon Sequencing, Targeted DNA Sequencing, Targeted RNA Sequencing |
Nucleic Acid Type | RNA | RNA, DNA |
Specialized Sample Types | Blood, FACS Cells, Fresh/Frozen Tissue | Blood, FACS Cells, FFPE Tissue, Fresh/Frozen Tissue |
Species Category | Human | Human |
Analysis with the AmpliSeq for Illumina Immune Repertoire Plus, TCR beta Panel Application Note
Application Note | HTML
Automated Solutions for AmpliSeq for Illumina Sequencing Panels
Application Note | HTML
AmpliSeq for Illumina Immune Repertoire Plus, TCR beta Panel Data Sheet
Data Sheet | HTML
AmpliSeq for Illumina Immune Repertoire Panel Consumables & Equipment Documentation
Illumina Adapter Sequences Documentation
AmpliSeq for Illumina Immune Repertoire Panel Checklist Documentation
AmpliSeq for Illumina Immune Repertoire Panel Reference Guide Documentation
AmpliSeq for Illumina Immune Repertoire Panel Documentation
Index Adapters Pooling Guide Documentation
Custom Protocol Selector
Generates customized, end-to-end instructions