AmpliSeq for Illumina TCR beta-SR (Short Read) Panel is a highly multiplexed targeted resequencing panel intended for use with multiple sample types, including formalin-fixed paraffin-embedded (FFPE) tumor samples. It is designed to measure T cell diversity and clonal expansion by sequencing T cell receptor (TCR) beta chain rearrangements. You can use a single pool of multiplex PCR primers, library reagents, and sample barcodes to generate libraries from DNA/RNA extracted from FFPE, whole blood, fresh-frozen tissue, or fluorescence activated cell sorting (FACS)-sorted cells.
Key features include:
The AmpliSeq for Illumina TCR beta-SR Panel is part of an integrated workflow that includes AmpliSeq for Illumina polymerase chain reaction (PCR)-based library preparation, Illumina sequencing by synthesis (SBS) chemistry and next-generation sequencing (NGS) technology, and automated analysis.
This ready-to-use panel saves you the time and effort of identifying targets, designing primers, and optimizing panels. It enables characterization of the hypervariable complementary determining region 3 (CDR3) of the TCR beta chain. This information can help immunology researchers understand immune function, autoimmune diseases, and immune-mediated adverse effects.
Learn More About AmpliSeq for Illumina| Instrument | Recommended Number of Samples | Read Length |
|---|---|---|
| MiniSeq System | Samples per run will depend on sample type. | 2 × 151 bp |
| MiSeq System | Samples per run will depend on sample type. | 2 × 151 bp |
| NextSeq 550 System | Samples per run will depend on sample type. | 2 × 151 bp |
| AmpliSeq for Illumina TCR beta-SR Panel | AmpliSeq for Illumina Immune Repertoire Plus, TCR beta Panel | |
|---|---|---|
| Assay Time | 5.5-7.5 hours | 5.5 – 7.5 hours (library prep only; doesn't include library quantification, normalization, or pooling time) |
| Content Specifications | Sequences TCR beta chain rearrangements, with up to 80 bp read-length amplicons for characterizing CDR3. | RNA evaluation of TCRβ chain rearrangements, including CDR1, CDR2, and CDR3 (with up to 400 bp read-length amplicons) |
| Hands-On Time | <1.5 hours | < 1.5 hours |
| Input Quantity | 1 ng | 10–1,000 ng |
| Method | Amplicon Sequencing, Targeted DNA Sequencing, Targeted RNA Sequencing | Amplicon Sequencing, Targeted RNA Sequencing |
| Nucleic Acid Type | RNA, DNA | RNA |
| Specialized Sample Types | Blood, FACS Cells, FFPE Tissue, Fresh/Frozen Tissue | Blood, FACS Cells, Fresh/Frozen Tissue |
| Species Category | Human | Human |
| System Compatibility | MiniSeq, MiSeq, MiSeq, NextSeq 1000, NextSeq 2000, NextSeq 550 | MiniSeq, MiSeq, MiSeq, NextSeq 1000, NextSeq 2000, NextSeq 550 |
Automated Solutions for AmpliSeq for Illumina Sequencing Panels
Application Note | HTML
Data Sheet | HTML | 5 versions
AmpliSeq for Illumina TCR beta-SR Panel Documentation
Custom Protocol Selector
Generates customized, end-to-end instructions
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