|Instrument||Recommended Number of Samples||Read Length|
|NextSeq 550 System||RNA Profiling: 13–40 samples per run (based on 10 million reads per
Transcriptome Analysis: 5–16 samples per run (based on 25 million reads per sample)
|2 x 75 bp
2 x 75 bp
|HiSeq 2500 System||RNA Profiling: 60–400 samples per run (dual flow cell; based on 10
million reads per sample)
Transcriptome Analysis: 24–160 samples per run (dual flow cell; based on 25 million reads per sample)
|2 x 75 bp
2 x 75 bp
|NovaSeq 6000 System||RNA Profiling (samples per run, dual flow cell): S1: 320, S2:384,
S4:768. Limited by index combinations (dual). Based on 10 million reads.
Transcriptome Analysis (samples per run, dual flow cell): S1: 128, S2: 256, S4: 768. Limited by index combinations (dual). Based on 25 million reads.
|≤ 2 × 100 bp
≤ 2 × 100 bp
|TruSeq Stranded mRNA||TruSeq RNA Library Prep Kit v2||TruSeq RNA Exome||SureCell WTA 3′ Library Prep Kit for the ddSEQ System|
|Assay Time||~10.5 hours||~10.5 hours||~2 Days||11-12 hours|
|Content Specifications||Captures the coding transcriptome with strand information||Captures the coding transcriptome (without strand information)||Captures the coding transcriptome/RNA exome||Captures the coding transcriptome (3' enriched), with strand-specific information|
|Description||Gives researchers a clear, comprehensive view of the coding transcriptome with precise strand information.||A simple, cost-effective research solution for analysis of the coding transcriptome.||Gives researchers a clear, comprehensive view of the coding transcriptome with precise strand information and a reduced sample input requirement.||Enables single-cell RNA-Seq studies using a droplet generator that isolates ~1200 cells per cartridge, and a simple library prep process.|
|Hands-On Time||~4.5 hours||~4.5 hours||~11 hours||4-5 hours|
|Input Quantity||0.1 – 1 ug total RNA or 10 - 100 ng previously isolated mRNA (from species with polyA tails)||0.1 - 1 ug total RNA or 10 - 400 ng previously isolated mRNA (from species with polyA tails)||10 ng total RNA from fresh/frozen samples, or 20 ng total RNA from FFPE samples||~45,000 cells per cartridge (required input)|
|Mechanism of Action||Oligo-dT beads capture polyA tails||Oligo-dT beads capture polyA tails||Biotinylated capture probes that target coding RNA. Does not require RNA with poly-A tails.||Oligo dT capture, single cell|
|Method||mRNA Sequencing||mRNA Sequencing||Exome Sequencing , mRNA Sequencing||mRNA Sequencing|
|Multiplexing||1–96||Up to 24-plex per lane||Up to 24 single, 96 combinatorial (CD) dual||1-24|
|Specialized Sample Types||Not FFPE-Compatible||Not FFPE-Compatible||FFPE Tissue, Low-Input Samples||Not FFPE-Compatible, Single Cells|
|Species Category||Bovine, Human, Mammalian, Mouse, Rat||Bovine, Human, Mammalian, Mouse, Rat||Human||Human, Mouse|
Researchers at the Icahn School of Medicine at Mount Sinai are studying mtDNA, piRNA, and TCR repertoire diversity to unlock the secrets of cancer, infertility, and autoimmune disease.Read More
RNA-Seq and HLA typing are increasing the power and efficiency of a target discovery platform.Read More
Figure 2 from TruSeq Stranded mRNA and Total RNA Library Prep Kits Data Sheet, December 2015
Technical replicates of FFPE tissue show high concordance, indicating robust library prep performance. Axes are log2(FPKM). R2 value is shown.
This product and its use are the subject of one or more issued and/or pending U.S. and foreign patent applications owned by Max Planck Gesellschaft, exclusively licensed to New England Biolabs, Inc. and sublicensed to Illumina, Inc. The purchase of this product from Illumina, Inc., its affiliates, or its authorized resellers and distributors conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product by the buyer (whether the buyer is an academic or for profit entity). The purchase of this product does not convey a license under any claims in the foregoing patents or patent applications direct to producing the product. The buyer cannot sell or otherwise transfer this product or its components to a third party or otherwise use the product for the following COMMERCIAL PURPOSES: (I) use of the product or its components in manufacturing; or (2) use of the product or its components for therapeutic or prophylactic purposes in human or animals.