TruSeq Stranded mRNA offers a streamlined, cost-efficient, and scalable solution for coding transcriptome analysis. It is compatible with a wide range of samples.
Get precise measurement of mRNA strand orientation for detection of antisense transcription, enhanced transcript annotation, and increased alignment efficiency. High coverage uniformity enhances the discovery of features such as alternative transcripts, gene fusions, and allele-specific expression.
Stranded information identifies from which of the 2 DNA strands a given RNA transcript was derived. This information provides increased confidence in transcript annotation, particularly for nonhuman samples. Identifying strand origin increases the percentage of reads that align, reducing sequencing costs per sample.
The workflow enables robust interrogation of both standard and low-quality samples, and workflows compatible with a wide range of study designs.
Prepare libraries for Illumina sequencing in less than a day. Illumina Qualified Methods are available on a range of automation platforms through our partners.
Read a comprehensive assessment of Illumina’s ribosomal RNA depletion protocol
|Instrument||Recommended Number of Samples||Read Length|
|NextSeq 550 System||RNA Profiling: 13–40 samples per run (based on 10 million reads per sample)
Transcriptome Analysis: 5–16 samples per run (based on 25 million reads per sample)
|2 x 75 bp
2 x 75 bp
|NovaSeq 6000 System||RNA Profiling (samples per run, dual flow cell): S1: 320, S2:384, S4:768. Limited by index combinations (dual). Based on 10 million reads.
Transcriptome Analysis (samples per run, dual flow cell): S1: 128, S2: 256, S4: 768. Limited by index combinations (dual). Based on 25 million reads.
|≤ 2 × 100 bp
≤ 2 × 100 bp
|TruSeq Stranded mRNA||Illumina Stranded mRNA Prep||Illumina RNA Prep with Enrichment|
|Assay Time||~10.5 hours||6.5 hours||< 9 hours|
|Automation Capability||Liquid Handling Robots||Liquid Handling Robots||Liquid Handling Robots|
|Content Specifications||Captures the coding transcriptome with strand information||Captures the coding transcriptome with strand information||Captures the coding transcriptome when used with Illumina Exome Panel|
|Description||Gives researchers a clear, comprehensive view of the coding transcriptome with precise strand information.||A simple, cost-effective solution for analysis of the coding transcriptome with precise strand information||A reproducible, economical solution enabling targeted transcript detection and discovery from a broad range of sample types and inputs including formalin-fixed, paraffin- embedded (FFPE) tissues and other low-quality samples|
|Hands-On Time||~4.5 hours||< 3 hours||< 2 hours|
|Input Quantity||0.1 – 1 ug total RNA or 10 - 100 ng previously isolated mRNA (from species with polyA tails)||25-1000 ng standard-quality total RNA||10ng total RNA from fresh/frozen samples, or 20ng total RNA from FFPE samples|
|Mechanism of Action||Oligo-dT beads capture polyA tails||PolyA capture, ligation-based addition of adapters and indexes||Bead-linked transposome|
|Method||mRNA Sequencing||mRNA Sequencing||mRNA Sequencing, Target Enrichment, Target Enrichment, Targeted RNA Sequencing|
|Multiplexing||1–96||Up to 384 Unique Dual Indexes (UDIs)||Up to 384 Unique Dual Indexes (UDIs)|
|Specialized Sample Types||Not FFPE-Compatible||Low-Input Samples, Not FFPE-Compatible||Blood, FFPE Tissue, Low-Input Samples|
|Species Category||Bovine, Human, Mammalian, Mouse, Rat||Bovine, Human, Mammalian, Mouse, Rat||Human, Virus|
The product previously known as the TruSeq Stranded mRNA Library Prep Kit (Cat. No. RS-122-2101, RS-122-2102, and RS-122-2103) is now called TruSeq Stranded mRNA. The product configuration has changed. In the new configuration, the library preparation and index adapters can be purchased separately. The list of components available for individual purchase can be found in the “Select Product(s)” section.
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Figure 2 from TruSeq Stranded mRNA and Total RNA Library Prep Kits Data Sheet, December 2015
Technical replicates of FFPE tissue show high concordance, indicating robust library prep performance. Axes are log2(FPKM). R2 value is shown.
Robust low-input RNA sequencing with TruSeq Stranded Total RNA Library Prep Gold
Application Note | HTML
Data Sheet | PDF | 5 versions
TruSeq Stranded mRNA Support - Documentation & Literature
TruSeq Stranded mRNA Consumables & Equipment Documentation
TruSeq Stranded mRNA Documentation
TruSeq Stranded mRNA Reference Guide Documentation
TruSeq Stranded mRNA Checklist Documentation
Custom Protocol Selector
Generates customized, end-to-end instructions
This product and its use are the subject of one or more issued and/or pending U.S. and foreign patent applications owned by Max Planck Gesellschaft, exclusively licensed to New England Biolabs, Inc. and sublicensed to Illumina, Inc. The purchase of this product from Illumina, Inc., its affiliates, or its authorized resellers and distributors conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product by the buyer (whether the buyer is an academic or for profit entity). The purchase of this product does not convey a license under any claims in the foregoing patents or patent applications direct to producing the product. The buyer cannot sell or otherwise transfer this product or its components to a third party or otherwise use the product for the following COMMERCIAL PURPOSES: (I) use of the product or its components in manufacturing; or (2) use of the product or its components for therapeutic or prophylactic purposes in human or animals.