TruSeq Targeted RNA Expression Library Prep Kits are now obsolete and are no longer available through MyIllumina. We recommend AmpliSeq for Illumina Custom RNA Panel as an alternative solution.
TruSeq Targeted RNA Expression Kits enable highly customizable mid- to high-plex gene expression profiling studies.
You can define panels of 12-1,000 assays to target individual exons, isoforms, splice junctions, coding SNPS (cSNPs), gene fusions, and non-coding RNA transcripts, plus multiplex up to 384 samples. Generate the data equivalent of up to 25,000 individual RT-PCR assays in a single run.*
Assays include convenient online panel design and ordering, a rapid and simple workflow starting with as little as 50 ng of RNA, and automated, on-instrument data analysis.
Order custom panels using DesignStudio, a free, user-friendly, web-based tool. Simply select the assays you need and add them to your order, with no design time.
Human Brain Reference RNA (HBRR), Universal Human Reference RNA (UHRR), and human liver RNA samples were prepared using the TruSeq Targeted RNA Expression Kit.
These libraries were sequenced on the MiniSeq System using the MiniSeq High Output Reagent Kit at a 1 x 51 bp read length configuration. The total yield was 2.04 Gb with 96.5% of bases at or above Q30.
Note that access to this data requires a BaseSpace Sequence Hub login.
Register for BaseSpace Sequence Hub
View TruSeq Targeted RNA Project Data
(secondary analysis with BaseSpace Apps)
*Refers to instruments with catalog number SY-410-1001 and upgrade, or catalog number SY-410-1003
| Instrument | Recommended Number of Samples | Read Length |
|---|---|---|
| MiniSeq System | Samples per run will depend on panel size. | 1 x 50 bp |
| MiSeq System | Samples per run will depend on panel size. | 1 x 50 bp |
| NextSeq 550 System | Samples per run will depend on panel size. | 1 x 50 bp |
Dr. Hughes at University of Missouri-St. Louis uses TruSeq Targeted RNA Expression to uncover the genetic mechanisms of circadian rhythms.
Learn MoreFrank Middleton, Ph.D., at SUNY-UMU developed a focused gene panel to screen for expression alterations caused by a newly discovered CNV in schizophrenia using the MiSeq System.
Learn More
Figure 3 from TruSeq Targeted RNA Expression Data Sheet, August 2013
With TruSeq RNA Expression, run 500 targets on 48 samples in one run in less than two days, compared to 63 runs in ~16 days with qPCR methods.
Considerations for Designing a Successful TruSeq Targeted RNA Expression Experiment
Technical Note | PDF < 1 MB
Expression Analysis of FFPE Samples
Technical Note | PDF 1 MB
TruSeq Targeted RNA Expression Data Sheet
Data Sheet | PDF < 1 MB
Illumina Experiment Manager User Guide Documentation
TruSeq Targeted RNA Expression Reference Guide Documentation
TruSeq Targeted RNA Expression Checklist Documentation
TruSeq Targeted RNA Expression Kit Documentation
TruSeq Targeted RNA Expression Consumables & Equipment Documentation
Custom Protocol Selector
Generates customized, end-to-end instructions
TruSeq Targeted RNA Expression fixed panels (including neurodegeneration, p53, apoptosis, stem cells, and others) have been discontinued. We recommend using TruSeq Targeted RNA Expression custom panels as a replacement. Illumina remains committed to providing you with high-quality support and service.
Provides comprehensive gene fusion detection in formalin-fixed, paraffin-embedded (FFPE) and other cancer research samples.
Prepare sequencing libraries for small genomes, PCR amplicons, plasmids, or cDNA in as little as 90 minutes, with a low DNA input requirement.
Targeted, custom RNA research panels optimized for sequencing up to 1200 genomic targets of interest.
Targeting 1385 cancer genes for gene expression, variant, and fusion detection studies in many RNA sample types including blood, bone marrow, and formalin-fixed, paraffin-embedded (FFPE) tissue.
Optimized chemistry to increase cluster density and read length, and improve sequencing quality scores, compared to earlier MiSeq reagent kit versions.
