TruSeq Targeted RNA Expression Library Prep Kits are now obsolete and are no longer available through MyIllumina. We recommend AmpliSeq for Illumina Custom RNA Panel as an alternative solution.

TruSeq Targeted RNA Expression Library Prep Kits

Targeted RNA assays for gene expression studies: custom sequencing panels with a simple workflow and automated data analysis. Read More...
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TruSeq Targeted RNA Custom Panel Kit (48 samples)


TruSeq Targeted RNA Custom Panel Kit (96 samples)


Accessory Products
What accessories do I need?

TruSeq Targeted RNA Index Kit (48 indexes, 48 samples)



TruSeq Targeted RNA Index Kit A (96 indexes, 384 samples)



TruSeq Targeted RNA Index Kit B (96 indexes, 384 samples)



TruSeq Targeted RNA Index Kit C (96 indexes, 384 samples)



TruSeq Targeted RNA Index Kit D (96 indexes, 384 samples)



Product Highlights

TruSeq Targeted RNA Expression Kits enable highly customizable mid- to high-plex gene expression profiling studies.

You can define panels of 12-1,000 assays to target individual exons, isoforms, splice junctions, coding SNPS (cSNPs), gene fusions, and non-coding RNA transcripts, plus multiplex up to 384 samples. Generate the data equivalent of up to 25,000 individual RT-PCR assays in a single run.*

Assays include convenient online panel design and ordering, a rapid and simple workflow starting with as little as 50 ng of RNA, and automated, on-instrument data analysis.

Types of Study Designs
  • Custom panels—select up to 1000 targets from a database of over 400,000 predesigned assays.
  • Add-on (supplemental) content—add a second oligo pool of up to 1000 targets to a previously designed TruSeq Targeted RNA Expression custom panel.
Start a Custom Project

Order custom panels using DesignStudio, a free, user-friendly, web-based tool. Simply select the assays you need and add them to your order, with no design time.

Log in to DesignStudio

Browse TruSeq Targeted RNA Sample Data

Human Brain Reference RNA (HBRR), Universal Human Reference RNA (UHRR), and human liver RNA samples were prepared using the TruSeq Targeted RNA Expression Kit.

These libraries were sequenced on the MiniSeq System using the MiniSeq High Output Reagent Kit at a 1 x 51 bp read length configuration. The total yield was 2.04 Gb with 96.5% of bases at or above Q30.

Note that access to this data requires a BaseSpace Sequence Hub login.

Register for BaseSpace Sequence Hub

View TruSeq Targeted RNA Project Data
(secondary analysis with BaseSpace Apps)

*Refers to instruments with catalog number SY-410-1001 and upgrade, or catalog number SY-410-1003

Frequently Purchased Together


Project Recommendations

Instrument Recommended Number of Samples Read Length
MiniSeq System Samples per run will depend on panel size. 1 x 50 bp
MiSeq System Samples per run will depend on panel size. 1 x 50 bp
NextSeq 550 System Samples per run will depend on panel size. 1 x 50 bp

Method-Specific Workflow Example


Customer Stories

Genomics Demystifies the Gears of Circadian Clocks

Dr. Hughes at University of Missouri-St. Louis uses TruSeq Targeted RNA Expression to uncover the genetic mechanisms of circadian rhythms.

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TruSeq Targeted RNA Expression on the MiSeq System Uncovers Effect of CNV Linked to Schizophrenia

Frank Middleton, Ph.D., at SUNY-UMU developed a focused gene panel to screen for expression alterations caused by a newly discovered CNV in schizophrenia using the MiSeq System.

Learn More

Supporting Data and Figures

TruSeq Targeted RNA vs qPCR Workflow
Supporting Data and Figures

Figure 3 from TruSeq Targeted RNA Expression Data Sheet, August 2013

With TruSeq RNA Expression, run 500 targets on 48 samples in one run in less than two days, compared to 63 runs in ~16 days with qPCR methods.


TruSeq Targeted RNA Expression fixed panels (including neurodegeneration, p53, apoptosis, stem cells, and others) have been discontinued. We recommend using TruSeq Targeted RNA Expression custom panels as a replacement. Illumina remains committed to providing you with high-quality support and service.

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