Illumina DNA Prep and Illumina DNA PCR-Free Prep are available. These library prep solutions offer improved performance while reducing turnaround time and hands-on time compared to TruSeq DNA Nano.

TruSeq DNA Nano

Generate whole-genome sequencing libraries and efficiently interrogate samples with limited available DNA. Read More...
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Library Prep

TruSeq Nano DNA Low Throughput Library Prep Kit (24 samples)

20015964

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TruSeq Nano DNA High Throughput Library Prep Kit (96 samples)

20015965

Price
 
 

Index Adapters

IDT for Illumina – TruSeq DNA UD Indexes v2 (96 Indexes, 96 Samples)

20040870

Price
 
 

Illumina Advantage Products

TG TruSeq® Nano DNA LT Sample Prep Kit, Set A (24 Samples)

TG-202-1001

Price
 
 

TG TruSeq® Nano DNA LT Sample Prep Kit, Set B (24 Samples)

TG-202-1002

Price
 
 

TG Truseq® Nano DNA Accessory Kit

TG-280-1001

Price
 
 

Product Highlights

TruSeq DNA Nano enables efficient interrogation of samples with limited available DNA. Based on mechanical fragmentation–ligation chemistry, this low-input method delivers high coverage quality and reduced bias for virtually any sequencing application.

  • Designed for low sample input
  • High coverage quality
  • Accelerated library prep
Manual preparation of high-quality libraries in less than a day

TruSeq DNA Nano has been streamlined by replacing gel-based size selection with bead-based selection, enabling researchers to prepare high-quality libraries in less than a day. It is optimized for a variety of read lengths, from 2 × 101 bp to 2 × 151 bp.

Use with limited DNA samples

TruSeq DNA Nano offers excellent results with as little as 100 ng input, eliminating the typical requirement for micrograms of DNA. This enables researchers to study samples with limited available DNA (eg, tumor samples) and helps preserve samples for use in future or alternate studies. In addition to accelerating the workflow, bead-based size selection avoids typical sample loss associated with gel-based selection.

Reduce library bias and coverage gaps

TruSeq DNA Nano reduces the number and average size of typical PCR-induced gaps in coverage. The enhanced workflow reduces library bias and improves coverage uniformity across the genome. These kits also provide excellent coverage of traditionally challenging genomic content, including GC-rich regions, promoters, and repetitive regions. This enables researchers to access more information from each sequencing run. Truseq DNA Nano products are validated for high-quality genomic coverage for virtually any next-generation sequencing application.

Access flexible throughput options
  • TruSeq DNA Nano with Single Indexes supports 24-sample manual processing for low-throughput (LT) studies.
  • TruSeq DNA Nano with 96 CD Indexes  supports 96-sample processing for high-throughput (HT) studies, and can be automated on liquid-handling robots (or processed manually).
  • IDT for Illumina Unique Dual (UD) Indexes (24 or 96) offer increased plexity that enables accurate assignment of reads and efficient use of the flow cell.
Fast, Automation-friendly Workflow

Prepare libraries for Illumina sequencing in less than a day. Illumina Qualified Methods are available on a range of automation platforms through our partners.

Frequently Purchased Together

Specifications

Project Recommendations

Instrument Recommended Number of Samples Read Length
NextSeq 550 System 1 sample per run (based on 30× coverage of a human genome) 350 bp insert: ≤ 2 × 100 bp
550 bp insert: ≤ 2 × 150 bp

Product Comparison

TruSeq DNA Nano TruSeq DNA PCR-Free Illumina DNA Prep
Assay Time ~6 hours total assay time 5 hours total assay time ~3-4 hours (from DNA extraction to normalized library)
Automation Capability Liquid Handling Robots Liquid Handling Robots Liquid Handling Robots
Description A low-input research method that delivers high genome coverage quality and reduced bias. A simple, all-inclusive PCR-free prep for whole-genome sequencing studies with the ability to sequence through challenging regions of the genome. A fast, flexible workflow for a wide range of research applications and sample types, from human to microbial whole-genome sequencing and more.
Hands-On Time ~4 hours 4 hours 1-1.5 hours
Input Quantity 100 ng genomic DNA 1 ug DNA Small genomes (e.g. microbial): 1-500 ng DNA. Large genomes (e.g. human): 100-500 ng DNA. (For blood and saliva, see the reference guide).
Mechanism of Action Mechanical DNA fragmentation and adapter ligation. Workflow uses reduced-bias PCR and is gel-free. Mechanical DNA fragmentation and adapter ligation. Workflow is PCR-free and gel-free. Bead-linked transposome
Method Genotyping by Sequencing, Shotgun Sequencing, Whole-Genome Sequencing Genotyping by Sequencing, Shotgun Sequencing, Whole-Genome Sequencing Amplicon Sequencing, De Novo Sequencing, Shotgun Sequencing, Whole-Genome Sequencing
Multiplexing Up to 24 single, 96 combinatorial (CD) dual, 24 unique dual and 96 unique dual (UD) combinations (available soon) Up to 24 single, 96 combinatorial (CD) dual, 24 unique dual and 96 unique dual (UD) combinations (available soon) Up to 384 unique dual (UD) combinations and 96 combinatorial dual (CD) combinations
Specialized Sample Types Low-Input Samples, Not FFPE-Compatible Not FFPE-Compatible Blood, Not FFPE-Validated, Saliva
Species Category Human, Mammalian, Mouse, Other, Plant, Rat Human, Mammalian, Mouse, Other, Plant, Rat Any Species, Bacteria, Drosophila, Human, Mammalian, Mouse, Nematode, Plant, Rat, Virus, Yeast, Zebrafish
Species Details Compatible with most large DNA genomes. Compatible with most large DNA genomes. Compatible with any species
Target Insert Size 350 bp or 550 bp 350 bp or 550 bp ~350bp

Method-Specific Workflow Example

 

Customer Stories

Unmasking the Viral Etiology of Cancer and Immune Disease

Karolinska Institutet researchers use Illumina sequencers to identify novel HPV types associated with non-melanoma skin cancers.

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