Nextera Flex for Enrichment products are now called Illumina DNA Prep with Enrichment. The (S) designation denotes a bead-linked transposome that generates an insert size of ~200bp. We recommend ordering the new Illumina DNA/RNA UD Indexes Sets A-D (20091654, 20091656, 20091658, 20091660). The IDT for Illumina DNA/RNA UD Indexes Sets A-D (20027213, 20027214,20042666, 20042667) are obsolete and the last order date is January 30, 2025, as announced in PON2023-1440.
Illumina DNA Prep with Enrichment
A fast, integrated workflow for a wide range of applications providing targeted resequencing for custom panels, fixed panels, and whole-exome enrichment.
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Index Adapters
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Illumina DNA Prep with Enrichment Training
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Product Highlights
Illumina DNA Prep with Enrichment is the fastest and most flexible targeted sequencing solution for DNA in the Illumina library prep portfolio:
- A time-saving solution that is 85% faster than standard Illumina library prep and enrichment
- Integrated DNA extraction protocols for blood and saliva
- Optimized performance using bead-based library prep chemistry for reliable results
Fast Library Prep and Enrichment Workflow
Illumina DNA Prep with Enrichment uses a fast, user-friendly workflow. On-bead tagmentation chemistry is combined with a simplified, single hybridization protocol to reduce total workflow time to ~6.5 hours. Go from DNA input and extraction to library normalization, enrichment, and post-enrichment amplification in just 6.5 hours. Total hands-on time is reduced to ~2 hours.
Integrated Sample Input
Illumina DNA Prep with Enrichment supports a broad DNA input range (50–1000 ng) and multiple sample types, including blood, saliva, genomic DNA, and formalin-fixed, paraffin-embedded (FFPE) tissue. Adding blood and saliva samples directly into the Illumina DNA Prep tagmentation reaction reduces the number of required quantification steps, saving time and money.
Optimized Performance
While accommodating various study requirements, the Illumina DNA Prep with Enrichment solution delivers consistent insert sizes, uniform coverage, and optimized performance, regardless of DNA input amount. The bead-based technology minimizes bias and opportunities for error, resulting in highly reproducible data across all Illumina sequencing systems.
Fast, Automation-friendly Workflow
Prepare libraries for Illumina sequencing in less than a day. Illumina Qualified Methods are available on a range of automation platforms through our partners.
Explore Illumina Library Prep
Discover the power that Illumina library prep tagmentation technology can bring to your lab through this interactive experience. Our library prep solutions offer whole-genome sequencing across various applications, flexibility across Illumina sequencers, and integrated sample input for blood and saliva without quantification.
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Frequently Purchased Together
Specifications
Project Recommendations
| Instrument | Recommended Number of Samples | Read Length |
|---|---|---|
| iSeq 100 System | TruSight Cancer: 4 samples per run (based on 280x mean targeted coverage; 99% target coverage at 20x) TruSight Cardio: 4 samples per run (based on 170x mean targeted coverage; 100% target coverage at 20x) |
2 x 100 bp 2 x 100 bp |
| MiniSeq System | TruSight Cancer (samples per run): mid output: 8, high output: 25 (based on 280x mean targeted coverage; 99% target coverage at 20x) TruSight Cardio (samples per run): mid output: 8, high output: 25 (based on 170x mean targeted coverage; 100% target coverage at 20x) |
2 x 100 bp 2 x 100 bp |
| MiSeq System | TruSight Cancer (samples per run): v2 reagents: 15, v3 reagents: 25 (based on 280x mean targeted coverage; 99% target coverage at 20x) TruSight Cardio (samples per run): v2 reagents: 15, v3 reagents: 25 (based on 170x mean targeted coverage; 100% target coverage at 20x) TruSight One (samples per run): v2 reagents: 1, v3 reagents: 3 (based on 120x mean targeted coverage; 95% target coverage at 20x) |
2 x 100 bp 2 x 100 bp 2 x 100 bp (up to 2 x 150 bp is possible) |
| NextSeq 550 System | Exome (samples per run): mid output: 5, high output: 16 (based on 50x mean targeted coverage; 90% target coverage at 20X) TruSight One (samples per run): mid output: 12, high output: 36 (based on 120x mean targeted coverage; 95% target coverage at 20x) TruSight One Expanded (samples per run): mid output: 7, high output: 24 (based on 130x mean targeted coverage; 97% target coverage at 20x) |
2 x 100 bp 2 x 100 bp (up to 2 x 150 bp is possible) 2 x 100 bp (up to 2 x 150 bp is possible) |
| HiSeq 4000 System | TruSight One Expanded (samples per run, single flow cell): 151 (based on 130x mean targeted coverage; 97% target coverage at 20x) | 2 x 100 bp |
| NovaSeq 6000 System | Exome (samples per run, by flow cell type): S1: 128, S2: 164, S4: 400 (based on 50x mean targeted coverage; 90% target coverage at 20X; S4 limited by available indexes) | 2 x 100 bp |
Case Studies
Tumor exomes reveal new insights into cancer biology
Dr. Paola Ulivi, Dr. Milena Urbini and Dr. Gianluca Tedali from the Translational Oncology Unit at IRST-IRCCS Bioscience Laboratory discuss how tumor exome sequencing enables a deeper understanding of the molecular landscape of cancers and guides biomarker discovery
Read InterviewProduct Literature
Tumor exome sequencing with Illumina DNA Prep with Enrichment and the DRAGEN Platform
Application Note | PDF 1 MB
Somatic Variant Detection in FFPE Samples with Nextera Flex for Enrichment
Application Note | HTML
Whole-Exome Sequencing with Nextera Flex for Enrichment
Application Note | HTML
Data Sheet | HTML | 7 versions
Illumina library preparation solutions
Brochure | PDF 2 MB
Application Note | PDF < 1 MB
NextSeq 550 exome sequencing solution
Application Note | PDF < 1 MB
NextSeq 1000 and NextSeq 2000 exome sequencing solution
Application Note | PDF < 1 MB
User-definable parameters in the Illumina DNA Prep with Enrichment workflow
Technical Note | PDF < 1 MB
Data Sheet | PDF | 5 versions
Manuals and Support Information
Illumina DNA Prep with Enrichment Reference Guide Documentation
Illumina Adapter Sequences Documentation
Illumina DNA Prep with Enrichment Checklist Documentation
Illumina DNA Prep with Enrichment Documentation
Urinary Pathogen ID/AMR Panel Documentation
Enrichment BaseSpace App Documentation
Illumina DNA Prep with Enrichment Consumables & Equipment Documentation
Urinary Pathogen ID/AMR Panel Reference Documentation
Index Adapters Pooling Guide Documentation
Custom Protocol Selector
Generates customized, end-to-end instructions
AmpliSeq for Illumina Focus Panel
Targeted DNA and RNA research panel investigating 52 genes with known relevance to solid tumors.
MiSeq Reagent Kit v3
Optimized chemistry to increase cluster density and read length, and improve sequencing quality scores, compared to earlier MiSeq reagent kit versions.
NextSeq 500/550 v2.5 Kits
These sequencing reagent kits offer increased stability and robustness over the v2 kit version.
TruSight Oncology 500
Assay targeting multiple variant types, including microsatellite instability (MSI) and tumor mutational burden (TMB).
Nextera XT DNA Library Preparation Kit
Prepare sequencing libraries for small genomes, PCR amplicons, plasmids, or cDNA in as little as 90 minutes, with a low DNA input requirement.
Illumina DNA PCR-Free Prep
A high-performing, fast, and integrated workflow for sensitive applications such as human whole-genome sequencing.
*Results from example data set. Actual performance may vary.