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Illumina DNA Prep with Enrichment

A fast, integrated workflow for a wide range of applications providing targeted resequencing for custom panels, fixed panels, and whole-exome enrichment. Read More...
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Illumina® DNA Prep with Enrichment, (S) Tagmentation (96 Samples)

20025524

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Illumina® DNA Prep with Enrichment, (S) Tagmentation (16 Samples)

20025523

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Illumina® DNA Prep, (S) Tagmentation (96 Samples)

20025520

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Illumina® DNA Prep, (S) Tagmentation (16 Samples)

20025519

Price
 
 
Panel

TruSight Cancer – Enrichment Oligos only (4 or 8 Enrichment Reactions)

FC-121-0202

Price
 
 

Illumina Exome Panel – Enrichment Oligos Only

20020183

Price
 
 

TruSight Hereditary Cancer – Enrichment Oligos Only (8 Enrichment Reactions)

20029551

Price
 
 

TruSight One – Enrichment Oligos only (6 Enrichment Reactions)

20029227

Price
 
 

TruSight One Expanded – Enrichment Oligos only (6 Enrichment Reactions)

20029226

Price
 
 

TruSight Cardio – Enrichment Oligos only (8 Enrichment Reactions)

20029229

Price
 
 

Illumina Custom Enrichment Panel (8 Enrichment Reactions)

20025371

Index Adapters

IDT® for Illumina® DNA/RNA UD Indexes Set A, Tagmentation (96 Indexes, 96 Samples)

20027213

Price
 
 

IDT® for Illumina® DNA/RNA UD Indexes Set B, Tagmentation (96 Indexes, 96 Samples)

20027214

Price
 
 

IDT® for Illumina® DNA/RNA UD Indexes Set C, Tagmentation (96 Indexes, 96 Samples)

20042666

Price
 
 

IDT® for Illumina® DNA/RNA UD Indexes Set D, Tagmentation (96 Indexes, 96 Samples)

20042667

Price
 
 
Services

Illumina DNA Prep with Enrichment Training

20028457

Accessory Products

Illumina Purification Bead, 100mL

20060057

Price
 
 

Illumina Purification Bead, 400mL

20060058

Price
 
 

Flex Lysis Reagent Kit (96 reactions)

20018706

Price
 
 

Infinium FFPE QC Kit (384 reactions)

WG-321-1001

Price
 
 

Infinium MIDI Heatblock Insert

BD-60-601

Price
 
 

Illumina® Free Adapter Blocking Reagent (12 Reactions)

20024144

Illumina® Free Adapter Blocking Reagent (48 Reactions)

20024145

Price
 
 
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Illumina DNA Prep with Enrichment

Product Highlights

Illumina DNA Prep with Enrichment is the fastest and most flexible targeted sequencing solution for DNA in the Illumina library prep portfolio:

  • A time-saving solution that is 85% faster than standard Illumina library prep and enrichment
  • Integrated DNA extraction protocols for blood and saliva
  • Optimized performance using bead-based library prep chemistry for reliable results
Fast Library Prep and Enrichment Workflow

Illumina DNA Prep with Enrichment uses a fast, user-friendly workflow. On-bead tagmentation chemistry is combined with a simplified, single hybridization protocol to reduce total workflow time to ~6.5 hours. Go from DNA input and extraction to library normalization, enrichment, and post-enrichment amplification in just 6.5 hours. Total hands-on time is reduced to ~2 hours.

Integrated Sample Input

Illumina DNA Prep with Enrichment supports a broad DNA input range (50–1000 ng) and multiple sample types, including blood, saliva, genomic DNA, and formalin-fixed, paraffin-embedded (FFPE) tissue. Adding blood and saliva samples directly into the Illumina DNA Prep tagmentation reaction reduces the number of required quantification steps, saving time and money.

Optimized Performance

While accommodating various study requirements, the Illumina DNA Prep with Enrichment solution delivers consistent insert sizes, uniform coverage, and optimized performance, regardless of DNA input amount. The bead-based technology minimizes bias and opportunities for error, resulting in highly reproducible data across all Illumina sequencing systems.

Fast, Automation-friendly Workflow

Prepare libraries for Illumina sequencing in less than a day. Illumina Qualified Methods are available on a range of automation platforms through our partners.

Explore Illumina Library Prep

Discover the power that Illumina library prep tagmentation technology can bring to your lab through this interactive experience. Our library prep solutions offer whole-genome sequencing across various applications, flexibility across Illumina sequencers, and integrated sample input for blood and saliva without quantification.

View Infographic
Introduction to Nextera DNA Flex Library Prep Kit

Frequently Purchased Together

Specifications

Project Recommendations

Instrument Recommended Number of Samples Read Length
iSeq 100 System TruSight Cancer: 4 samples per run (based on 280x mean targeted coverage; 99% target coverage at 20x)
TruSight Cardio: 4 samples per run (based on 170x mean targeted coverage; 100% target coverage at 20x)		 2 x 100 bp

2 x 100 bp
MiniSeq System TruSight Cancer (samples per run): mid output: 8, high output: 25 (based on 280x mean targeted coverage; 99% target coverage at 20x)
TruSight Cardio (samples per run): mid output: 8, high output: 25 (based on 170x mean targeted coverage; 100% target coverage at 20x)		 2 x 100 bp

2 x 100 bp
MiSeq System TruSight Cancer (samples per run): v2 reagents: 15, v3 reagents: 25 (based on 280x mean targeted coverage; 99% target coverage at 20x)
TruSight Cardio (samples per run): v2 reagents: 15, v3 reagents: 25 (based on 170x mean targeted coverage; 100% target coverage at 20x)
TruSight One (samples per run): v2 reagents: 1, v3 reagents: 3 (based on 120x mean targeted coverage; 95% target coverage at 20x)		 2 x 100 bp

2 x 100 bp

2 x 100 bp (up to 2 x 150 bp is possible)
NextSeq 550 System Exome (samples per run): mid output: 5, high output: 16 (based on 50x mean targeted coverage; 90% target coverage at 20X)

TruSight One (samples per run): mid output: 12, high output: 36 (based on 120x mean targeted coverage; 95% target coverage at 20x)
TruSight One Expanded (samples per run): mid output: 7, high output: 24 (based on 130x mean targeted coverage; 97% target coverage at 20x)		 2 x 100 bp
2 x 100 bp (up to 2 x 150 bp is possible)
2 x 100 bp (up to 2 x 150 bp is possible)
HiSeq 4000 System TruSight One Expanded (samples per run, single flow cell): 151 (based on 130x mean targeted coverage; 97% target coverage at 20x) 2 x 100 bp
NovaSeq 6000 System Exome (samples per run, by flow cell type): S1: 128, S2: 164, S4: 400 (based on 50x mean targeted coverage; 90% target coverage at 20X; S4 limited by available indexes) 2 x 100 bp

Method-Specific Workflow Example

 
Prepare Library for:
Whole Exome Sequencing
Targeted Resequencing
 
Sequence Efficiently with:
iSeq 100 System
MiniSeq System
MiSeq System
NextSeq 550 System
NextSeq 2000 System
NovaSeq 6000 System
 
Analyze Your Data with:
DRAGEN Germline or Somatic Pipelines on the DRAGEN Server or the DRAGEN Enrichment App on BSSH
 

Product Literature

Tumor exome sequencing with Illumina DNA Prep with Enrichment and the DRAGEN Platform

Application Note | PDF 1 MB

Somatic Variant Detection in FFPE Samples with Nextera Flex for Enrichment

Application Note | HTML

Whole-Exome Sequencing with Nextera Flex for Enrichment

Application Note | HTML

Fast, Flexible, and Self-Normalizing Library Prep with Nextera Flex Chemistry

Application Note | HTML

Data Sheet | HTML | 7 versions

Illumina library preparation solutions

Brochure | PDF 2 MB

NextSeq 550 exome sequencing solution

Application Note | PDF < 1 MB

NextSeq 1000 and NextSeq 2000 exome sequencing solution

Application Note | PDF < 1 MB

User-definable parameters in the Illumina DNA Prep with Enrichment workflow

Technical Note | PDF < 1 MB

Data Sheet | PDF | 5 versions

Manuals and Support Information

Illumina DNA Prep with Enrichment Reference Guide Documentation

Illumina Adapter Sequences Documentation

Illumina DNA Prep with Enrichment Checklist Documentation

Illumina DNA Prep with Enrichment Documentation

Urinary Pathogen ID/AMR Panel Documentation

Enrichment BaseSpace App Documentation

Illumina DNA Prep with Enrichment Consumables & Equipment Documentation

Urinary Pathogen ID/AMR Panel Reference Documentation

Index Adapters Pooling Guide Documentation

Custom Protocol Selector
Generates customized, end-to-end instructions

Related Products

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*Results from example data set. Actual performance may vary.

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For Research Use Only

Not for use in diagnostic procedures (except as specifically noted).

Innovative technologies

At Illumina, our goal is to apply innovative technologies to the analysis of genetic variation and function, making studies possible that were not even imaginable just a few years ago. It is mission critical for us to deliver innovative, flexible, and scalable solutions to meet the needs of our customers. As a global company that places high value on collaborative interactions, rapid delivery of solutions, and providing the highest level of quality, we strive to meet this challenge. Illumina innovative sequencing and array technologies are fueling groundbreaking advancements in life science research, translational and consumer genomics, and molecular diagnostics.

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