The Nextera Rapid Capture Exome and Expanded Exome Kits will be discontinued on May 1, 2018, with a final shipment date of June 1, 2018. Nextera DNA Exome is the recommended replacement product. Illumina remains committed to providing you with high-quality support and service.

Nextera Rapid Capture Exome and Expanded Exome Kits (to be discontinued)

Identify coding variants up to 70% faster with this exome library prep and enrichment kit. Expanded kit sequences untranslated regions (UTRs) and miRNA.Read More...
Select Product(s)

Nextera Rapid Capture Exome (8 rxn x 1 plex)

FC-140-1000

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Nextera Rapid Capture Exome (2 rxn x 12 plex)

FC-140-1001

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Nextera Rapid Capture Exome (4 rxn x 12 plex)

FC-140-1002

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Nextera Rapid Capture Exome (8 rxn x 12 plex)

FC-140-1003

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Nextera Rapid Capture Exome (8 rxn x 3 plex)

FC-140-1083

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Nextera Rapid Capture Exome (8 rxn x 6 plex)

FC-140-1086

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Nextera Rapid Capture Exome (8 rxn x 9 plex)

FC-140-1089

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Nextera Rapid Capture Expanded Exome (2 rxn x 12 plex)

FC-140-1004

Nextera Rapid Capture Expanded Exome (4 rxn x 12 plex)

FC-140-1005

Nextera Rapid Capture Expanded Exome (8 rxn x 12 plex)

FC-140-1006

Product Highlights

The Nextera Rapid Capture Exome and Expanded Exome are all-in-one kits for library preparation and exome enrichment that allow researchers to identify coding variants up to 70% faster than other methods. This optimized exome delivers 45 Mb of expertly selected exonic content and requires as little as 4 Gb of sequencing.

Extend coverage to UTRs and miRNA with Expanded Exome

Nextera Rapid Capture Expanded Exome delivers 62 Mb of genomic content, including exons, untranslated regions (UTRs), and miRNA. This kit features a highly optimized probe set that delivers comprehensive coverage of exonic sequence.

Illumina provides example data sets to demonstrate the high uniformity and accuracy of the Nextera Rapid Capture Exome library prep kit. View the exome sequencing data.

Add on custom content to make Nextera Rapid Capture Exome even more powerful

Researchers can define custom content for this workflow using Nextera Rapid Capture Custom Enrichment. This custom assay allows researchers to rapidly interrogate those portions of the human genome most important to their specific research. Choose from a fully custom design or start from a defined content set, such as Nextera Rapid Capture Exome, and include areas of interest using add-on functionality. Learn More.

Find an up-to-date list of high-throughput automation vendors with robotic systems compatible with this library preparation kit

Specifications

Method-Specific Workflow Example

 

Customer Stories

New Method Enables Analysis of Preserved Cancer Specimens with Exome Sequencing

A novel QC approach and the Nextera Rapid Capture Exome Kit enable researchers to sequence degraded DNA from FFPE tumor samples successfully.

Learn More
Looking Beyond Single-Drug Approaches to Precision Oncology Research

Researchers rely on transcriptomics to uncover multiple drug-susceptible tumorigenic pathways.

Learn More
Comparison of Illumina Exome Sequencing Solutions

TruSeq Rapid Exome and TruSeq Exome Library Preparation Kits are compatible with a variety of sample types, including FFPE, and deliver a seamless workflow, from sample to push-button data analysis.

Metric Nextera Rapid Capture Exome TruSeq Rapid Exome TruSeq Exome
DNA input 50 ng 50 ng 100 ng
Sample types DNA DNA DNA and FFPE
Hands-on time 5 hours 3 hours 6 hours
Total assay time 1.5 days 1 day 2.5 days
Hyb time 16 hours 2 hours 16 hours
On-target % 50% 75% 80%
% coverage at 20x at 8Gb 80% 88% 90%

Related Products

Nextera DNA Exome

This exome sequencing library preparation solution uses a simple, one-day workflow to provide a fast path to greater understanding of the human exome.


TruSeq DNA Exome

This kit provides a low-cost exome sequencing solution that delivers exceptional target coverage over a broad range of read depths.


Nextera Rapid Capture Custom Enrichment Kit

Rapidly interrogate those portions of the human genome most important to your specific research.